6jps
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Human H chain ferritin mutant-MBP== | |
| + | <StructureSection load='6jps' size='340' side='right'caption='[[6jps]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jps]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JPS FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jps OCA], [http://pdbe.org/6jps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jps RCSB], [http://www.ebi.ac.uk/pdbsum/6jps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jps ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/Q6NS36_HUMAN Q6NS36_HUMAN]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.[RuleBase:RU361145] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein nanocages have recently received considerable attention in the fields of nanoscience and nanomedicine and have been used as either biotemplates for the preparation of a variety of nanomaterials or vehicles for drugs or imaging agents. However, their utilization for detection of heavy metal ions has yet to be explored. In this study, by grafting a mercury binding peptide (MBP) on the exterior surface of a recombinant human H-chain ferritin (rHuHF) nanocage, we successfully prepared a new protein nanocage (HuHF-MBP) which exhibits high binding capacity and affinity for Hg2+. The fluorescence of HuHF-MBP labeled with a green fluorescent dye fluorescein isothiocyanate (FITC) can be quenched by graphene oxide (GO), while addition of Hg2+ to the above solution recovered the quenched fluorescence in a dose-dependent manner. Thus, this system consisting of FITC-labeled HuHF-MBP and GO, where FITC and graphene oxide were used as fluorescent reporter probes, has great potential to be explored as a sensor for Hg2+ detection. Indeed, this newly constructed protein sensor exhibited high sensitivity and selectivity for Hg2+, and the limit of detection was 1.0 nM. The construction of this system provides an alternative strategy for the preparation of heavy metal ion sensors by using protein nanocages as biotemplates. | ||
| - | + | Re-designing ferritin nanocages for mercuric ion detection.,Wang Y, Chen H, Zang J, Zhang X, Zhao G Analyst. 2019 Sep 23;144(19):5890-5897. doi: 10.1039/c9an01110b. PMID:31497803<ref>PMID:31497803</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6jps" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen, H]] | ||
| + | [[Category: Wang, Y]] | ||
| + | [[Category: Zang, J]] | ||
| + | [[Category: Ferritin]] | ||
| + | [[Category: Mercuric ion binding peptide]] | ||
| + | [[Category: Metal binding protein]] | ||
Revision as of 05:17, 10 October 2019
Human H chain ferritin mutant-MBP
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