6s9s
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Dimerization domain of Xenopus laevis LDB1 in complex with darpin 10== | |
| + | <StructureSection load='6s9s' size='340' side='right'caption='[[6s9s]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6s9s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9S FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9s OCA], [http://pdbe.org/6s9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9s RCSB], [http://www.ebi.ac.uk/pdbsum/6s9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/LDB1_XENLA LDB1_XENLA]] Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. Acts as a coactivator together with otx2 to stimulate lhx1/lim1-mediated activation of the gsc promoter in the Spemann organizer. Acts synergistically with lhx1/lim1 and ssbp in axis formation.<ref>PMID:10926781</ref> <ref>PMID:12381786</ref> <ref>PMID:8918878</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Chip/LIM-domain binding protein (LDB)-single-stranded DNA-binding protein (SSDP) (ChiLS) complex controls numerous cell-fate decisions in animal cells, by mediating transcription of developmental control genes via remote enhancers. ChiLS is recruited to these enhancers by lineage-specific LIM-domain proteins that bind to its Chip/LDB subunit. ChiLS recently emerged as the core module of the Wnt enhanceosome, a multiprotein complex that primes developmental control genes for timely Wnt responses. ChiLS binds to NPFxD motifs within Pygopus (Pygo) and the Osa/ARID1A subunit of the BAF chromatin remodeling complex, which could synergize with LIM proteins in tethering ChiLS to enhancers. Chip/LDB and SSDP both contain N-terminal dimerization domains that constitute the bulk of their structured cores. Here, we report the crystal structures of these dimerization domains, in part aided by DARPin chaperones. We conducted systematic surface scanning by structure-designed mutations, followed by in vitro and in vivo binding assays, to determine conserved surface residues required for binding between Chip/LDB, SSDP, and Pygo-NPFxD. Based on this, and on the 4:2 (SSDP-Chip/LDB) stoichiometry of ChiLS, we derive a highly constrained structural model for this complex, which adopts a rotationally symmetrical SSDP2-LDB2-SSDP2 architecture. Integrity of ChiLS is essential for Pygo binding, and our mutational analysis places the NPFxD pockets on either side of the Chip/LDB dimer, each flanked by an SSDP dimer. The symmetry and multivalency of ChiLS underpin its function as an enhancer module integrating Wnt signals with lineage-specific factors to operate context-dependent transcriptional switches that are pivotal for normal development and cancer. | ||
| - | + | Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome.,Renko M, Fiedler M, Rutherford TJ, Schaefer JV, Pluckthun A, Bienz M Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1912705116. doi:, 10.1073/pnas.1912705116. PMID:31570581<ref>PMID:31570581</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6s9s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bienz, M]] | ||
| + | [[Category: Pluckthun, A]] | ||
| + | [[Category: Renko, M]] | ||
| + | [[Category: Schaefer, J V]] | ||
| + | [[Category: Gene regulation]] | ||
| + | [[Category: Wnt enhanceosome]] | ||
| + | [[Category: Wnt signalling]] | ||
Revision as of 05:26, 10 October 2019
Dimerization domain of Xenopus laevis LDB1 in complex with darpin 10
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