6chj
From Proteopedia
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==Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8== | ==Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8== | ||
| - | <StructureSection load='6chj' size='340' side='right' caption='[[6chj]], [[Resolution|resolution]] 2.43Å' scene=''> | + | <StructureSection load='6chj' size='340' side='right'caption='[[6chj]], [[Resolution|resolution]] 2.43Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6chj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6chj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Marhv Marhv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHJ FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Maqu_0168 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=351348 MARHV])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [http://pdbe.org/6chj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [http://www.ebi.ac.uk/pdbsum/6chj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [http://pdbe.org/6chj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [http://www.ebi.ac.uk/pdbsum/6chj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Wax esters are high-value products whose enzymatic synthesis is of increasing biotechnological interest. The fabrication of cell factories that mass-produce wax esters may provide a facile route towards a sustainable, and environment-friendly approach to a large-scale process for this commodity chemical. An expedient route for wax-ester biocatalysis may be facilitated by the action of enzymes termed wax ester synthases/diacylglycerol acyltransferases (WS/DGAT), which produce wax esters using fatty acids and alcohols as a precursor. In this work, we report the structure for a member of the WS/DGAT superfamily. The structural data in conjunction with bioinformatics and mutational analyses allowed us to identify the substrate binding pockets, and residues that may be important for catalysis. Using this information as a guide, we generated a mutant with preference towards shorter acyl-substrates. This study demonstrates the efficacy of a structure-guided engineering effort towards a WS/DGAT variant with preference towards wax esters of desired lengths. | ||
| + | |||
| + | Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.,Petronikolou N, Nair SK ACS Catal. 2018 Jul 6;8(7):6334-6344. doi: 10.1021/acscatal.8b00787. Epub 2018, Jun 1. PMID:31559109<ref>PMID:31559109</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6chj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Diacylglycerol O-acyltransferase]] | [[Category: Diacylglycerol O-acyltransferase]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Marhv]] | ||
[[Category: Petronikolou, N]] | [[Category: Petronikolou, N]] | ||
[[Category: Satish, S K]] | [[Category: Satish, S K]] | ||
Revision as of 05:59, 10 October 2019
Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8
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