3bsq

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Revision as of 10:33, 30 April 2008

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Template:STRUCTURE 3bsq

Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli

Overview

Human kallikrein 7, a major protease of human skin, has been synthesized directly in its native conformation in Escherichia coli by forcing the secretion of the newly synthesized polypeptide into the bacterial periplasm. The procedure yields a stable kallikrein 7 with highly specific activity that is inhibited efficiently by its specific inhibitor LEKTI domain 6. The protein was crystallized, and its three-dimensional structure was solved in the absence of protease inhibitors. The structure obtained agrees with that reported recently for human tissue kallikrein 7 crystallized in the presence of protease inhibitors from a preparation obtained in a baculovirus protein expression system. A model of the interaction between the protease and its inhibitor is proposed on the basis of both the three-dimensional structure of human tissue kallikrein 7 reported here and that of the LEKTI domain 6 solved previously by NMR.

About this Structure

3BSQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6)., Fernandez IS, Standker L, Magert HJ, Forssmann WG, Gimenez-Gallego G, Romero A, J Mol Biol. 2008 Apr 11;377(5):1488-97. Epub 2008 Feb 9. PMID:18329042 Page seeded by OCA on Wed Apr 30 13:33:12 2008