6i2z
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Isolated globin domain of the Bordetella pertussis globin-coupled sensor== | |
| + | <StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uiq|4uiq]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [http://pdbe.org/6i2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [http://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | AIMS: Structural and functional characterization of the globin coupled sensors (GCS) of <i>Azotobacter vinelandii </i>(AvGReg) and <i>Bordetella pertussis</i> (BpeGReg). RESULTS: UV/Vis and RR spectroscopies confirm the presence in <i>Av</i>GReg and <i>Bpe</i>GReg of a globin domain capable of reversible gaseous ligand binding. In <i>Av</i>GReg an influence of the transmitter domain on the heme proximal region of the globin domain can be seen, and <i>k'</i>CO is higher than for other GCSs. The O<sub>2</sub> binding kinetics suggest the presence of an open and a closed conformation. As for <i>Bpe</i>GReg, the fully oxygenated <i>Av</i>GReg show a very high DGC activity. The CO rebinding to <i>Bpe</i>GReg indicates that intra- and intermolecular interactions influence the ligand binding. The globin domains of both proteins (<i>Av</i>GReg-Gb and <i>Bpe</i>Greg-Gb*) share the same GCS fold, a similar proximal but a different distal side structure. They homodimerize through a G-H helical bundle as in other GCS. However, <i>Bpe</i>GReg-Gb* shows also a second dimerization mode. INNOVATION: This paper extends our knowledge on the GCS proteins and contributes to a better understanding of the GCSs' role in the formation of bacterial biofilms. CONCLUSIONS: <i>Av</i>GReg and <i>Bpe</i>Greg conform to the GCS family, share a similar overall structure but they have different properties in terms of the ligand binding. In particular, <i>Av</i>Greg shows an open and closed conformation that in the latter form will very tightly bind oxygen. <i>Bpe</i>GReg has only one closed conformation. In both proteins, it is the fully oxygenated GCS form that catalyzes the production of the second messenger. | ||
| - | + | Structural and functional characterization of the globin-coupled sensors of <i>Azotobacter vinelandii </i>and <i>Bordetella pertussis</i>.,Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835<ref>PMID:31559835</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6i2z" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Berghmans, H]] | [[Category: Berghmans, H]] | ||
| + | [[Category: Bolognesi, M]] | ||
[[Category: Bruno, S]] | [[Category: Bruno, S]] | ||
[[Category: Cuypers, B]] | [[Category: Cuypers, B]] | ||
| - | [[Category: De Schutter, A]] | ||
[[Category: Dewilde, S]] | [[Category: Dewilde, S]] | ||
| - | [[Category: | + | [[Category: Doorslaer, S Van]] |
| - | [[Category: | + | [[Category: Germani, F]] |
| + | [[Category: Hauwaert, M L.Van]] | ||
[[Category: Moens, L]] | [[Category: Moens, L]] | ||
| + | [[Category: Mozzarelli, A]] | ||
| + | [[Category: Pesce, A]] | ||
| + | [[Category: Schutter, A De]] | ||
| + | [[Category: Biofilm formation]] | ||
| + | [[Category: C-di-gmp and enzyme specificity]] | ||
| + | [[Category: Heme-based sensor]] | ||
| + | [[Category: Oxygen affinity]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 05:49, 16 October 2019
Isolated globin domain of the Bordetella pertussis globin-coupled sensor
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Categories: Large Structures | Berghmans, H | Bolognesi, M | Bruno, S | Cuypers, B | Dewilde, S | Doorslaer, S Van | Germani, F | Hauwaert, M L.Van | Moens, L | Mozzarelli, A | Pesce, A | Schutter, A De | Biofilm formation | C-di-gmp and enzyme specificity | Heme-based sensor | Oxygen affinity | Transferase
