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5o3j

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Revision as of 06:51, 16 October 2019

Crystal structure of TIA-1 RRM2 in complex with RNA

Structural highlights

5o3j is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	TIA1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TIA1_HUMAN] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Publication Abstract from PubMed

Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. RNA binding proteins (RBPs) represent an important class of multi-domain proteins, which regulate gene expression by recognizing linear or structured RNA sequence motifs. Here, we employ segmental perdeuteration of the three RNA recognition motif (RRM) domains in the RBP TIA-1 using Sortase A mediated protein ligation. We show that domain-selective perdeuteration combined with contrast-matched small-angle neutron scattering (SANS), SAXS and computational modeling provides valuable information to precisely define relative domain arrangements. The approach is generally applicable to study conformational arrangements of individual domains in multi-domain proteins and changes induced by ligand binding.

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.,Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9322-9325. doi: 10.1002/anie.201702904., Epub 2017 Jul 5. PMID:28636238^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M. Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9322-9325. doi: 10.1002/anie.201702904., Epub 2017 Jul 5. PMID:28636238 doi:http://dx.doi.org/10.1002/anie.201702904

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5o3j"

@@ Line 1: / Line 1: @@
 ==Crystal structure of TIA-1 RRM2 in complex with RNA==
-<StructureSection load='5o3j' size='340' side='right' caption='[[5o3j]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
+<StructureSection load='5o3j' size='340' side='right'caption='[[5o3j]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O3J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O3J FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [http://pdbe.org/5o3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [http://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [http://pdbe.org/5o3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [http://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Hennig, J]]
 [[Category: Jagtap, P K.A]]

5o3j

From Proteopedia

Revision as of 06:51, 16 October 2019

Crystal structure of TIA-1 RRM2 in complex with RNA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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