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5mjs

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Revision as of 07:42, 23 October 2019

S. pombe microtubule copolymerized with GTP and Mal3-143

Structural highlights

5mjs is a 9 chain structure with sequence from Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	nda3, alp12, SPBC26H8.07c (Fission yeast), mal3, SPAC18G6.15 (Fission yeast), nda2, SPBC16A3.15c (Fission yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TBB_SCHPO] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [TBA1_SCHPO] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [MAL3_SCHPO] May play a role in regulating the integrity of microtubules possibly by influencing their stability. Involved in an anchoring mechanism to maintain tea2 and tip1 at growing microtubule ends. Strongly stimulates the ATPase activity of tea2.^[1] ^[2]

Publication Abstract from PubMed

Using cryo-electron microscopy, we characterize the architecture of microtubules assembled from Schizosaccharomyces pombe tubulin, in the presence and absence of their regulatory partner Mal3. Cryo-electron tomography reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interprotofilament contacts, with protofilaments skewed around the microtubule axis. Copolymerization with Mal3 favors 13 protofilament microtubules with reduced protofilament skew, indicating that Mal3 adjusts interprotofilament interfaces. A 4.6-A resolution structure of microtubule-bound Mal3 shows that Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal lattice compaction associated with EB protein binding and GTP hydrolysis. Our results firmly support a structural plasticity view of microtubule dynamics in which microtubule lattice conformation is sensitive to a variety of effectors and differently so for different tubulins.

Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.,von Loeffelholz O, Venables NA, Drummond DR, Katsuki M, Cross R, Moores CA Nat Commun. 2017 Dec 13;8(1):2110. doi: 10.1038/s41467-017-02241-5. PMID:29235477^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Busch KE, Hayles J, Nurse P, Brunner D. Tea2p kinesin is involved in spatial microtubule organization by transporting tip1p on microtubules. Dev Cell. 2004 Jun;6(6):831-43. PMID:15177031 doi:http://dx.doi.org/10.1016/j.devcel.2004.05.008
↑ Browning H, Hackney DD. The EB1 homolog Mal3 stimulates the ATPase of the kinesin Tea2 by recruiting it to the microtubule. J Biol Chem. 2005 Apr 1;280(13):12299-304. Epub 2005 Jan 23. PMID:15665379 doi:http://dx.doi.org/10.1074/jbc.M413620200
↑ von Loeffelholz O, Venables NA, Drummond DR, Katsuki M, Cross R, Moores CA. Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics. Nat Commun. 2017 Dec 13;8(1):2110. doi: 10.1038/s41467-017-02241-5. PMID:29235477 doi:http://dx.doi.org/10.1038/s41467-017-02241-5

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mjs"

@@ Line 1: / Line 1: @@
 ==S. pombe microtubule copolymerized with GTP and Mal3-143==
-<StructureSection load='5mjs' size='340' side='right' caption='[[5mjs]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
+<StructureSection load='5mjs' size='340' side='right'caption='[[5mjs]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mjs]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mjs]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJS FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nda3, alp12, SPBC26H8.07c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), mal3, SPAC18G6.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), nda2, SPBC16A3.15c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mjs OCA], [http://pdbe.org/5mjs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mjs RCSB], [http://www.ebi.ac.uk/pdbsum/5mjs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mjs ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Fission yeast]]
+[[Category: Large Structures]]
 [[Category: Loeffelholz, O von]]
 [[Category: Moores, C]]
 [[Category: Schizosaccharomyces pombe microtubule]]
 [[Category: Structural protein]]

5mjs

From Proteopedia

Revision as of 07:42, 23 October 2019

S. pombe microtubule copolymerized with GTP and Mal3-143

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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