6hjy
From Proteopedia
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<StructureSection load='6hjy' size='340' side='right'caption='[[6hjy]], [[Resolution|resolution]] 2.78Å' scene=''> | <StructureSection load='6hjy' size='340' side='right'caption='[[6hjy]], [[Resolution|resolution]] 2.78Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6hjy]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hjy]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"erwinia_carotovora_var._chrysanthemi"_(burkholder_et_al._1953)_dye_1969 "erwinia carotovora var. chrysanthemi" (burkholder et al. 1953) dye 1969] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJY FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjy OCA], [http://pdbe.org/6hjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hjy RCSB], [http://www.ebi.ac.uk/pdbsum/6hjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjy ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjy OCA], [http://pdbe.org/6hjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hjy RCSB], [http://www.ebi.ac.uk/pdbsum/6hjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phospholipids are key components of cellular membranes and are emerging as important functional regulators of different membrane proteins, including pentameric ligand-gated ion channels (pLGICs). Here, we take advantage of the prokaryote channel ELIC (Erwinia ligand-gated ion channel) as a model to understand the determinants of phospholipid interactions in this family of receptors. A high-resolution structure of ELIC in a lipid-bound state reveals a phospholipid site at the lower half of pore-forming transmembrane helices M1 and M4 and at a nearby site for neurosteroids, cholesterol or general anesthetics. This site is shaped by an M4-helix kink and a Trp-Arg-Pro triad that is highly conserved in eukaryote GABAA/C and glycine receptors. A combined approach reveals that M4 is intrinsically flexible and that M4 deletions or disruptions of the lipid-binding site accelerate desensitization in ELIC, suggesting that lipid interactions shape the agonist response. Our data offer a structural context for understanding lipid modulation in pLGICs. | ||
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| + | A lipid site shapes the agonist response of a pentameric ligand-gated ion channel.,Henault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan G, Nury H, Steyaert J, Baenziger JE, Ulens C Nat Chem Biol. 2019 Oct 7. pii: 10.1038/s41589-019-0369-4. doi:, 10.1038/s41589-019-0369-4. PMID:31591563<ref>PMID:31591563</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6hjy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Camelus glama]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Evans, G L]] | [[Category: Evans, G L]] | ||
Revision as of 07:54, 23 October 2019
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) Delta8 truncation mutant in complex with nanobody 72
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