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6fhy
From Proteopedia
(Difference between revisions)
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==Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside== | ==Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside== | ||
| - | <StructureSection load='6fhy' size='340' side='right' caption='[[6fhy]], [[Resolution|resolution]] 1.86Å' scene=''> | + | <StructureSection load='6fhy' size='340' side='right'caption='[[6fhy]], [[Resolution|resolution]] 1.86Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43950 Atcc 43950]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHY FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DS8:(2~{S},3~{S},4~{R},5~{S},6~{S})-2-[[(2~{S},4~{R},6~{R})-2-(hydroxymethyl)-6-oxidanyl-oxan-4-yl]methyl]-6-methyl-oxane-3,4,5-triol'>DS8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DS8:(2~{S},3~{S},4~{R},5~{S},6~{S})-2-[[(2~{S},4~{R},6~{R})-2-(hydroxymethyl)-6-oxidanyl-oxan-4-yl]methyl]-6-methyl-oxane-3,4,5-triol'>DS8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PAU_00698 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=291112 ATCC 43950])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhy OCA], [http://pdbe.org/6fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhy RCSB], [http://www.ebi.ac.uk/pdbsum/6fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhy ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhy OCA], [http://pdbe.org/6fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhy RCSB], [http://www.ebi.ac.uk/pdbsum/6fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A recently described bangle lectin (PHL) from the bacterium Photorhabdus asymbiotica was identified as a mainly fucose-binding protein that could play an important role in the host-pathogen interaction and in the modulation of host immune response. Structural studies showed that PHL is a homo-dimer that contains up to seven L-fucose-specific binding sites per monomer. For these reasons, potential ligands of the PHL lectin: alpha-L-fucopyranosyl-containing mono-, di-, tetra-, hexa- and dodecavalent ligands were tested. Two types of polyvalent structures were investigated - calix[4]arenes and dendrimers. The shared feature of all these structures was a C-glycosidic bond instead of the more common but physiologically unstable O-glycosidic bond. The inhibition potential of the tested structures was assessed using different techniques - hemagglutination, surface plasmon resonance, isothermal titration calorimetry, and cell cross-linking. All the ligands proved to be better than free L-fucose. The most active hexavalent dendrimer exhibited affinity three orders of magnitude higher than that of standard L-fucose. To determine the binding mode of some ligands, crystal complex PHL/fucosides 2 - 4 were prepared and studied using X-ray crystallography. The electron density in complexes proved the presence of the compounds in 6 out of 7 fucose-binding sites. | ||
| + | |||
| + | Fucosylated inhibitors of recently identified bangle lectin from Photorhabdus asymbiotica.,Paulikova G, Houser J, Kasakova M, Oroszova B, Bertolotti B, Parkan K, Moravcova J, Wimmerova M Sci Rep. 2019 Oct 17;9(1):14904. doi: 10.1038/s41598-019-51357-9. PMID:31624296<ref>PMID:31624296</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6fhy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 43950]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Houser, J]] | [[Category: Houser, J]] | ||
[[Category: Jancarikova, G]] | [[Category: Jancarikova, G]] | ||
Current revision
Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside
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