6is8
From Proteopedia
(Difference between revisions)
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<StructureSection load='6is8' size='340' side='right'caption='[[6is8]], [[Resolution|resolution]] 1.68Å' scene=''> | <StructureSection load='6is8' size='340' side='right'caption='[[6is8]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6is8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IS8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6is8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IS8 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6is8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is8 OCA], [http://pdbe.org/6is8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is8 RCSB], [http://www.ebi.ac.uk/pdbsum/6is8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6is8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is8 OCA], [http://pdbe.org/6is8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is8 RCSB], [http://www.ebi.ac.uk/pdbsum/6is8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Holliday junction (HJ) is a key intermediate during homologous recombination and DNA double-strand break repair. Timely HJ resolution by resolvases is critical for maintaining genome stability. The mechanisms underlying sequence-specific substrate recognition and cleavage by resolvases remain elusive. The monokaryotic chloroplast 1 protein (MOC1) specifically cleaves four-way DNA junctions in a sequence-specific manner. Here, we report the crystal structures of MOC1 from Zea mays, alone or bound to HJ DNA. MOC1 uses a unique beta-hairpin to embrace the DNA junction. A base-recognition motif specifically interacts with the junction center, inducing base flipping and pseudobase-pair formation at the strand-exchanging points. Structures of MOC1 bound to HJ and different metal ions support a two-metal ion catalysis mechanism. Further molecular dynamics simulations and biochemical analyses reveal a communication between specific substrate recognition and metal ion-dependent catalysis. Our study thus provides a mechanism for how a resolvase turns substrate specificity into catalytic efficiency. | ||
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| + | Structural basis of sequence-specific Holliday junction cleavage by MOC1.,Lin H, Zhang D, Zuo K, Yuan C, Li J, Huang M, Lin Z Nat Chem Biol. 2019 Oct 14. pii: 10.1038/s41589-019-0377-4. doi:, 10.1038/s41589-019-0377-4. PMID:31611704<ref>PMID:31611704</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6is8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Maize]] | ||
[[Category: Lin, H]] | [[Category: Lin, H]] | ||
[[Category: Lin, Z]] | [[Category: Lin, Z]] | ||
Revision as of 09:32, 30 October 2019
Crystal structure of ZmMoc1 D115N mutant in complex with Holliday junction
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