Methylcitrate synthase

From Proteopedia

Revision as of 09:59, 30 October 2019

spinqualitylabelsall models Methylcitrate synthase dimer complex with glycerol (PDB entry 3o8j) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Methylcitrate synthase (MCS) participates in propanoate metabolism. It catalyzes the conversion of acyl to alkyl group in the reaction which converts propanoyl-CoA and oxaloacetate to 2-hydroxybutane-1,2,3-tricarboxylate and CoA[1]. The metabolism of propionyl-CoA in microorganism is performed by the methylcitrate cycle. The methylcitrate cycle contains MCS, methylcitrate dehydrogenase and methylisocitrate lyase[2]. The methylcitrate cycle can serve as a carbon and energy source of growth. In the presence of glucose, the cycle serves to remove the toxic propionate. Relevance The high levels of propionate used to preserve foodstuffs lead to fungal growth inhibition via the accumulation of intermediates of the methylcitrate cycle[3]. See also Citrate Synthase.

3D structures of methylcitrate synthase

Updated on 30-October-2019

3hwk – MCS – Mycobacterium tuberculosis
3o8j – MCS – Salmonella enterica
3tqg – MCS – Coxiella burnetii
5uqo, 5uqq, 5uqr – Af2-MCS – Aspergillus fumigata
5uqu, 6bom, 6bop – Af2-MCS (mutant)
6bol – Af2-MCS (mutant) + oxaloacetate
6bon, 6boo – Af2-MCS + oxaloacetate + CoA

References

1. ↑ Horswill AR, Escalante-Semerena JC. Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle. J Bacteriol. 1999 Sep;181(18):5615-23. PMID:10482501
2. ↑ Upton AM, McKinney JD. Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis. Microbiology. 2007 Dec;153(Pt 12):3973-82. PMID:18048912 doi:http://dx.doi.org/10.1099/mic.0.2007/011726-0
3. ↑ Brock M, Fischer R, Linder D, Buckel W. Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent. Mol Microbiol. 2000 Mar;35(5):961-73. PMID:10712680