1uvk

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[[Image:1uvk.gif|left|200px]]
[[Image:1uvk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1uvk |SIZE=350|CAPTION= <scene name='initialview01'>1uvk</scene>, resolution 2.45&Aring;
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The line below this paragraph, containing "STRUCTURE_1uvk", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CA1:Mn+Binding+Site+For+Chain+C'>CA1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1uvk| PDB=1uvk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uvk OCA], [http://www.ebi.ac.uk/pdbsum/1uvk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uvk RCSB]</span>
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}}
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'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX'''
'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX'''
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[[Category: Salgado, P S.]]
[[Category: Salgado, P S.]]
[[Category: Stuart, D I.]]
[[Category: Stuart, D I.]]
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[[Category: oligonucleotide]]
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[[Category: Oligonucleotide]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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[[Category: rna-dependent rna polymerase]]
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[[Category: Rna-dependent rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:56:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:15:52 2008''
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Revision as of 10:56, 30 April 2008

Image:1uvk.gif

Template:STRUCTURE 1uvk

THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX


Overview

The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.

About this Structure

1UVK is a Protein complex structure of sequences from Pseudomonas phage phi6. Full crystallographic information is available from OCA.

Reference

The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391 Page seeded by OCA on Wed Apr 30 13:56:23 2008

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