NADPH dehydrogenase

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== Function ==
== Function ==
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'''NADPH dehydrogenase''' or '''old yellow enzyme''' (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor<ref>PMID:12803488</ref>. See [[NAD(P)H]]. The acceptor is a quinone. OYE is a flavoenzyme with two cofactors: FAD and FMN.
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'''NADPH dehydrogenase''' or '''old yellow enzyme''' (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor<ref>PMID:12803488</ref>. See [[NAD(P)H]]. The acceptor is a quinone. OYE is a flavoenzyme with two cofactors: FAD and FMN. '''NADPH dehydrogenase quinone 1''' (NQO1) reduces quinones to hydroquinones.
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== Structural highlights ==
== Structural highlights ==
<scene name='59/595221/Cv/5'>OYE cofactor FMN interacts with the ligand in active site</scene><ref>PMID:11668181</ref>. Water molecules are shown as red spheres.
<scene name='59/595221/Cv/5'>OYE cofactor FMN interacts with the ligand in active site</scene><ref>PMID:11668181</ref>. Water molecules are shown as red spheres.
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**[[5v4p]] – yOYE 3 + FMN + p-hydroxybenzaldehyde <br />
**[[5v4p]] – yOYE 3 + FMN + p-hydroxybenzaldehyde <br />
**[[5v4v]] – yOYE 3 + FMN + nicotinamide <br />
**[[5v4v]] – yOYE 3 + FMN + nicotinamide <br />
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*NADPH dehydrogenase quinone 1
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**[[5a4k]] – hNQO1 (mutant) + FAD - human <br />
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**[[5fuq]], [[4cet]] – hNQO1 (mutant) + FAD + dicoumarol <br />
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**[[4cf6]] – hNQO1 (mutant) + FAD + cibacron blue <br />
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Revision as of 08:51, 6 November 2019

Structure of yeast NADPH dehydrogenase complex with FMN, p-hydroxybenzaldehyde and Mg+2 ion (green) (PDB code 1k03).

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3D Structures of NADPH dehydrogenase

Updated on 06-November-2019

References

  1. Ryu JY, Suh KH, Chung YH, Park YM, Chow WS, Park YI. NADPH dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light. Mol Cells. 2003 Apr 30;15(2):240-4. PMID:12803488
  2. Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V. The role of glutamine 114 in old yellow enzyme. J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181 doi:10.1074/jbc.M108453200

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Michal Harel, Alexander Berchansky

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