133d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:133d.jpg|left|200px]] | [[Image:133d.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_133d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_133d| PDB=133d | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''THE CRYSTAL STRUCTURE OF N4-METHYLCYTOSINE.GUANOSIN BASE-PAIRS IN THE SYNTHETIC HEXANUCLEOTIDE D(CGCGM(4)CG)''' | '''THE CRYSTAL STRUCTURE OF N4-METHYLCYTOSINE.GUANOSIN BASE-PAIRS IN THE SYNTHETIC HEXANUCLEOTIDE D(CGCGM(4)CG)''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=133D OCA]. | |
==Reference== | ==Reference== | ||
The crystal structure of N4-methylcytosine.guanosine base-pairs in the synthetic hexanucleotide d(CGCGm4CG)., Cervi AR, Guy A, Leonard GA, Teoule R, Hunter WN, Nucleic Acids Res. 1993 Dec 11;21(24):5623-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8284207 8284207] | The crystal structure of N4-methylcytosine.guanosine base-pairs in the synthetic hexanucleotide d(CGCGm4CG)., Cervi AR, Guy A, Leonard GA, Teoule R, Hunter WN, Nucleic Acids Res. 1993 Dec 11;21(24):5623-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8284207 8284207] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Cervi, A R.]] | [[Category: Cervi, A R.]] | ||
[[Category: Guy, A.]] | [[Category: Guy, A.]] | ||
| Line 29: | Line 25: | ||
[[Category: Leonard, G A.]] | [[Category: Leonard, G A.]] | ||
[[Category: Teoule, R.]] | [[Category: Teoule, R.]] | ||
| - | [[Category: | + | [[Category: Double helix]] |
| - | [[Category: | + | [[Category: Modified]] |
| - | [[Category: | + | [[Category: Z-dna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:28:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:28, 2 May 2008
THE CRYSTAL STRUCTURE OF N4-METHYLCYTOSINE.GUANOSIN BASE-PAIRS IN THE SYNTHETIC HEXANUCLEOTIDE D(CGCGM(4)CG)
Overview
The structure of d(CGCGm4CG) were m4C = N4-methylcytosine has been determined by crystallographic methods. The crystals are multifaced prisms, with orthorhombic space group P2(1)2(1)2(1) and unit cell dimensions of a = 17.98, b = 30.77 and c = 44.75A. The asymmetric unit consists of one duplex of hexanucleotide and 49 waters. The R-factor is 0.189 for 1495 reflections with F > or = sigma(F) to a resolution limit of 1.8A. The double helix has a Z-DNA type structure which appears to be intermediate in structure to the two previously characterised structure types for Z-DNA hexamers. The two m4C.G base-pairs adopt structures that are very similar to those of the equivalent base-pairs in the structure of the native sequence d(CGCGCG) except for the presence of the methyl groups which are trans to the N3 atoms of their parent nucleotides and protrude into the solvent region. The introduction of the modified base-pairs into the d(CGCGCG) duplex appears to have a minimal effect on the overall base-pair morphology of the Z-DNA duplex.
About this Structure
Full crystallographic information is available from OCA.
Reference
The crystal structure of N4-methylcytosine.guanosine base-pairs in the synthetic hexanucleotide d(CGCGm4CG)., Cervi AR, Guy A, Leonard GA, Teoule R, Hunter WN, Nucleic Acids Res. 1993 Dec 11;21(24):5623-9. PMID:8284207 Page seeded by OCA on Fri May 2 09:28:56 2008
Categories: Cervi, A R. | Guy, A. | Hunter, W N. | Leonard, G A. | Teoule, R. | Double helix | Modified | Z-dna
