4my5
From Proteopedia
(Difference between revisions)
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==Crystal structure of the aromatic amino acid aminotransferase from Streptococcus mutants== | ==Crystal structure of the aromatic amino acid aminotransferase from Streptococcus mutants== | ||
| - | <StructureSection load='4my5' size='340' side='right' caption='[[4my5]], [[Resolution|resolution]] 2.19Å' scene=''> | + | <StructureSection load='4my5' size='340' side='right'caption='[[4my5]], [[Resolution|resolution]] 2.19Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4my5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strmu Strmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MY5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4my5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strmu Strmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MY5 FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4my5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4my5 OCA], [http://pdbe.org/4my5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4my5 RCSB], [http://www.ebi.ac.uk/pdbsum/4my5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4my5 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4my5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4my5 OCA], [http://pdbe.org/4my5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4my5 RCSB], [http://www.ebi.ac.uk/pdbsum/4my5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4my5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptococcus mutans, a facultatively aerobic and Gram-positive bacterium, is the primary causative agent of dental caries and contributes to the multispecies biofilm known as dental plaque. In this study, the aromatic-amino-acid aminotransferase from Streptococcus mutans (SmAroAT) was recombinantly expressed in Escherichia coli. An effective purification protocol was established. The recombinant protein was crystallized using the hanging-drop vapor-diffusion method with PEG 3350 as the primary precipitant. The crystal structure of SmAroAT was solved at 2.2 A resolution by the molecular-replacement method. Structural analysis indicated that the proteins of the aromatic-amino-acid aminotransferase family have conserved structural elements that might play a role in substrate binding. These results may help in obtaining a better understanding of the catabolism and biosynthesis of aromatic amino acids. | ||
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| + | Crystal structure of the aromatic-amino-acid aminotransferase from Streptococcus mutans.,Cong X, Li X, Li S Acta Crystallogr F Struct Biol Commun. 2019 Feb 1;75(Pt 2):141-146. doi:, 10.1107/S2053230X18018472. Epub 2019 Jan 24. PMID:30713166<ref>PMID:30713166</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4my5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Strmu]] | [[Category: Strmu]] | ||
[[Category: Cong, X]] | [[Category: Cong, X]] | ||
Revision as of 15:58, 20 November 2019
Crystal structure of the aromatic amino acid aminotransferase from Streptococcus mutants
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