6u04
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of human BRPF1 PZP bound to histone H3 tail== | |
| + | <StructureSection load='6u04' size='340' side='right'caption='[[6u04]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6u04]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U04 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u04 OCA], [http://pdbe.org/6u04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u04 RCSB], [http://www.ebi.ac.uk/pdbsum/6u04 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u04 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The assembly of human histone acetyltransferase MOZ/MORF complexes relies on the scaffolding bromodomain plant homeodomain (PHD) finger 1 (BRPF1) subunit. The PHD-zinc-knuckle-PHD module of BRPF1 (BRPF1PZP) has been shown to associate with the histone H3 tail and DNA; however, the molecular mechanism underlying recognition of H3 and the relationship between the histone and DNA-binding activities remain unclear. In this study, we report the crystal structure of BRPF1PZP bound to the H3 tail and characterize the role of the bipartite interaction in the engagement of BRPF1PZP with the nucleosome core particle (NCP). We find that although both interactions of BRPF1PZP with the H3 tail and DNA are required for tight binding to NCP and for acetyltransferase function of the BRPF1-MORF-ING5-MEAF6 complex, binding to extranucleosomal DNA dominates. Our findings suggest that functionally active BRPF1PZP might be important in stabilization of the MOZ/MORF complexes at chromatin with accessible DNA. | ||
| - | + | Molecular Basis for the PZP Domain of BRPF1 Association with Chromatin.,Klein BJ, Cox KL, Jang SM, Cote J, Poirier MG, Kutateladze TG Structure. 2019 Nov 6. pii: S0969-2126(19)30355-7. doi:, 10.1016/j.str.2019.10.014. PMID:31711755<ref>PMID:31711755</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6u04" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Klein, B J]] | ||
| + | [[Category: Kutateladze, T G]] | ||
| + | [[Category: Dna binding]] | ||
| + | [[Category: Epigenetic]] | ||
| + | [[Category: Nucleosome]] | ||
| + | [[Category: Transcription]] | ||
Revision as of 07:01, 27 November 2019
Crystal structure of human BRPF1 PZP bound to histone H3 tail
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