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5l35

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Revision as of 07:49, 27 November 2019

Cryo-EM structure of bacteriophage Sf6 at 2.9 Angstrom resolution

Structural highlights

5l35 is a 7 chain structure with sequence from Shigella phage sf6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The enormous prevalence of tailed DNA bacteriophages on this planet is enabled by highly efficient self-assembly of hundreds of protein subunits into highly stable capsids. These capsids can stand with an internal pressure as high as approximately 50 atmospheres as a result of the phage DNA-packaging process. Here we report the complete atomic model of the headful DNA-packaging bacteriophage Sf6 at 2.9 A resolution determined by electron cryo-microscopy. The structure reveals the DNA-inflated, tensed state of a robust protein shell assembled via noncovalent interactions. Remarkable global conformational polymorphism of capsid proteins, a network formed by extended N arms, mortise-and-tenon-like intercapsomer joints, and abundant beta-sheet-like mainchain:mainchain intermolecular interactions, confers significant strength yet also flexibility required for capsid assembly and DNA packaging. Differential formations of the hexon and penton are mediated by a drastic alpha-helix-to-beta-strand structural transition. The assembly scheme revealed here may be common among tailed DNA phages and herpesviruses.

Structure of a headful DNA-packaging bacterial virus at 2.9 A resolution by electron cryo-microscopy.,Zhao H, Li K, Lynn AY, Aron KE, Yu G, Jiang W, Tang L Proc Natl Acad Sci U S A. 2017 Mar 20. pii: 201615025. doi:, 10.1073/pnas.1615025114. PMID:28320961^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao H, Li K, Lynn AY, Aron KE, Yu G, Jiang W, Tang L. Structure of a headful DNA-packaging bacterial virus at 2.9 A resolution by electron cryo-microscopy. Proc Natl Acad Sci U S A. 2017 Mar 20. pii: 201615025. doi:, 10.1073/pnas.1615025114. PMID:28320961 doi:http://dx.doi.org/10.1073/pnas.1615025114

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5l35"

@@ Line 1: / Line 1: @@
 ==Cryo-EM structure of bacteriophage Sf6 at 2.9 Angstrom resolution==
-<StructureSection load='5l35' size='340' side='right' caption='[[5l35]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
+<StructureSection load='5l35' size='340' side='right'caption='[[5l35]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5l35]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_phage_sf6 Shigella phage sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L35 FirstGlance]. <br>
@@ Line 20: / Line 20: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Shigella phage sf6]]
 [[Category: Tang, L]]

5l35

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Revision as of 07:49, 27 November 2019

Cryo-EM structure of bacteriophage Sf6 at 2.9 Angstrom resolution

Structural highlights

Publication Abstract from PubMed

References

Contents

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