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Glutaminyl-tRNA synthetase or GlnRS is a class 1 tRNA synthetase that requires tRNA binding for adenylate synthesis, which suggests that the large substrate could be required to form the active site structure. <ref name=Aminoacyl>12737824</ref> Genetic evidence shows that induced fit conformational changes are a big part in the mechanism of GlnRS. <ref name=Aminoacyl>12737824</ref> Glutaminyl-tRNA synthetase is an enzyme which is able to catalyze the transfer of the amino acid glutamine to the A76 hydroxyl group of tRNA^Gln. <ref name=Aminoacyl>12737824</ref> | Glutaminyl-tRNA synthetase or GlnRS is a class 1 tRNA synthetase that requires tRNA binding for adenylate synthesis, which suggests that the large substrate could be required to form the active site structure. <ref name=Aminoacyl>12737824</ref> Genetic evidence shows that induced fit conformational changes are a big part in the mechanism of GlnRS. <ref name=Aminoacyl>12737824</ref> Glutaminyl-tRNA synthetase is an enzyme which is able to catalyze the transfer of the amino acid glutamine to the A76 hydroxyl group of tRNA^Gln. <ref name=Aminoacyl>12737824</ref> | ||
== Structure == | == Structure == | ||
| - | The crystal structure of ligand-free E. coli glutaminyl-tRNA synthetase at 2.4 A shows substrate binding is needed to construct a catalytically proficient active site. <ref name=Aminoacyl>12737824</ref> The backbone atoms of 94 amino acid residues, <scene name='82/824625/Backbone_atoms_amino_acid_comp/1'>25–31, 36–63, 77–99, 213–239, and 252–260</scene> could be superimposed on their counterparts showing similarities close to each other. <ref name=Aminoacyl>12737824</ref> | + | The crystal structure of ligand-free E. coli glutaminyl-tRNA synthetase at 2.4 A shows substrate binding is needed to construct a catalytically proficient active site. <ref name=Aminoacyl>12737824</ref> The backbone atoms of 94 amino acid residues, <scene name='82/824625/Backbone_atoms_amino_acid_comp/1'>25–31, 36–63, 77–99, 213–239, and 252–260</scene> could be superimposed on their counterparts showing similarities close to each other. <ref name=Aminoacyl>12737824</ref> One of the amino acids in the <scene name='82/824625/Loop_compromising_amino_acids/1'>surface loop compromising amino acids 64-76</scene> of the dinucleotide fold adopts a new arrangement in the unligand enzyme. <ref name=Aminoacyl>12737824</ref> |
== Mutations == | == Mutations == | ||
Mutations in the coding of glutaminyl-tRNA synthetase can cause seizures especially in infants. <ref name=Atrophy>24656866</ref> Progressive microcephaly is a condition that involves mutations in gene coding of brain survival. <ref name=Atrophy>24656866</ref> | Mutations in the coding of glutaminyl-tRNA synthetase can cause seizures especially in infants. <ref name=Atrophy>24656866</ref> Progressive microcephaly is a condition that involves mutations in gene coding of brain survival. <ref name=Atrophy>24656866</ref> | ||
Revision as of 21:08, 29 November 2019
| This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594). |
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Glutaminyl-tRNA Synthetase
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References
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