Sandbox Reserved 1580

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (17:34, 2 December 2019) (edit) (undo)
 
Line 5: Line 5:
== Overview ==
== Overview ==
-
Glutaminyl-tRNA synthetase or GlnRS is a class 1 tRNA synthetase that requires tRNA binding for adenylate synthesis, which suggests that the large substrate could be required to form the active site structure. <ref name=frank>12737824</ref> Genetic evidence shows that induced fit conformational changes are a big part in the mechanism of GlnRS. <ref name=frank>PMID:12737824</ref> Glutaminyl-tRNA synthetase is an enzyme who's function is to catalyze the transfer of the amino acid glutamine to the A76 hydroxyl group of tRNA^Gln. <ref name=frank /> Glutaminyl-tRNA synthetase was highly expressed in the developing of unborn-baby related, human brains in many cell types. <ref name=Atrophy>PMID:24656866</ref>
+
Glutaminyl-tRNA synthetase or GlnRS is a class 1 tRNA synthetase that requires tRNA binding for adenylate synthesis, which suggests that the large substrate could be required to form the active site structure. <ref name=frank>PMID:12737824</ref> Genetic evidence shows that induced fit conformational changes are a big part in the mechanism of GlnRS. <ref name=frank /> Glutaminyl-tRNA synthetase is an enzyme who's function is to catalyze the transfer of the amino acid glutamine to the A76 hydroxyl group of tRNA^Gln. <ref name=frank /> Glutaminyl-tRNA synthetase was highly expressed in the developing of unborn-baby related, human brains in many cell types. <ref name=Atrophy>PMID:24656866</ref>
== Structure ==
== Structure ==
The crystal structure of ligand-free E. coli glutaminyl-tRNA synthetase at 2.4 A shows substrate binding is needed to construct a catalytically proficient active site. <ref name=frank /> Five hundred and twenty-one of the five hundred and thirty-three amino acids were able to be built into the electron density maps. <ref name=frank /> The backbone atoms of 94 amino acid residues, <scene name='82/824625/Backbone_atoms_amino_acid_comp/1'>25–31, 36–63, 77–99, 213–239, and 252–260</scene> could be superimposed on their counterparts showing similarities close to each other. <ref name=frank /> When superimposed there were few differences the unligand enzyme and the tRNA-bound enzyme.<ref name=frank /> One of the amino acids in the <scene name='82/824625/Loop_compromising_amino_acids/1'>surface loop compromising amino acids 64-76</scene> of the dinucleotide fold adopts a new arrangement in the unligand enzyme. <ref name=frank /> Also, two amino acids in different surface loops in C-terminal beta barrel regions are fully disordered, just like the surface loop compromising amino acids. <ref name=frank />
The crystal structure of ligand-free E. coli glutaminyl-tRNA synthetase at 2.4 A shows substrate binding is needed to construct a catalytically proficient active site. <ref name=frank /> Five hundred and twenty-one of the five hundred and thirty-three amino acids were able to be built into the electron density maps. <ref name=frank /> The backbone atoms of 94 amino acid residues, <scene name='82/824625/Backbone_atoms_amino_acid_comp/1'>25–31, 36–63, 77–99, 213–239, and 252–260</scene> could be superimposed on their counterparts showing similarities close to each other. <ref name=frank /> When superimposed there were few differences the unligand enzyme and the tRNA-bound enzyme.<ref name=frank /> One of the amino acids in the <scene name='82/824625/Loop_compromising_amino_acids/1'>surface loop compromising amino acids 64-76</scene> of the dinucleotide fold adopts a new arrangement in the unligand enzyme. <ref name=frank /> Also, two amino acids in different surface loops in C-terminal beta barrel regions are fully disordered, just like the surface loop compromising amino acids. <ref name=frank />

Current revision

This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594).
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • Click the 3D button (when editing, above the wikitext box) to insert Jmol.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing. For an example of a student Proteopedia page, please see Photosystem II, Tetanospasmin, or Guanine riboswitch.

Glutaminyl-tRNA Synthetase

Glutaminyl-tRNA synthetase

Drag the structure with the mouse to rotate

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Sherlin LD, Perona JJ. tRNA-dependent active site assembly in a class I aminoacyl-tRNA synthetase. Structure. 2003 May;11(5):591-603. PMID:12737824
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Zhang X, Ling J, Barcia G, Jing L, Wu J, Barry BJ, Mochida GH, Hill RS, Weimer JM, Stein Q, Poduri A, Partlow JN, Ville D, Dulac O, Yu TW, Lam AT, Servattalab S, Rodriguez J, Boddaert N, Munnich A, Colleaux L, Zon LI, Soll D, Walsh CA, Nabbout R. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet. 2014 Apr 3;94(4):547-58. doi: 10.1016/j.ajhg.2014.03.003. Epub, 2014 Mar 20. PMID:24656866 doi:http://dx.doi.org/10.1016/j.ajhg.2014.03.003
Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_1580"
Personal tools