6jox
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==triosephosphate isomerase-scylla paramamosain== | |
| + | <StructureSection load='6jox' size='340' side='right'caption='[[6jox]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jox]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JOX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JOX FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jox OCA], [http://pdbe.org/6jox PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jox RCSB], [http://www.ebi.ac.uk/pdbsum/6jox PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jox ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The triosephosphate isomerase (TIM), Scy p 8, is a crab allergen and shows cross-reactivity in the shellfish. Here, recombinant Scy p 8 was expressed, and its crystal structure was determined at a resolution of 1.8 A. The three-dimensional structure of Scy p 8 is primarily composed of a (beta/alpha)8-barrel motif prototype. Additionally, Scy p 8 showed cross-reactivity with high sequential and secondary structural identity among TIMs from shellfish species. The site-directed mutagenesis of critical amino acids of conformational epitopes was carried out, and the mutants of Trp 168 and Lys 237 to Ala reduced immunoglobulin E (IgE)-binding activity by approximately 30%, compared with wild-type TIM in an inhibition ELISA; however, it still induced basophil activation despite the interpatient variability between patients. These results can help to provide an accurate template for the analysis of the IgE binding and establish meaningful relationships between structure and allergenicity. | ||
| - | + | Crystal Structure Analysis and Conformational Epitope Mutation of Triosephosphate Isomerase, a Mud Crab Allergen.,Xia F, Li MS, Liu QM, Liu M, Yang Y, Cao MJ, Chen GX, Jin T, Liu GM J Agric Food Chem. 2019 Nov 20;67(46):12918-12926. doi: 10.1021/acs.jafc.9b05279., Epub 2019 Nov 11. PMID:31668066<ref>PMID:31668066</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6jox" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Triose-phosphate isomerase]] | ||
[[Category: Jin, T]] | [[Category: Jin, T]] | ||
[[Category: Xia, F]] | [[Category: Xia, F]] | ||
| + | [[Category: Allergen]] | ||
| + | [[Category: Blue crab]] | ||
| + | [[Category: Food allergy]] | ||
| + | [[Category: Isomerase]] | ||
| + | [[Category: Triosephosphate isomerase]] | ||
Revision as of 08:05, 4 December 2019
triosephosphate isomerase-scylla paramamosain
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