6kks
From Proteopedia
(Difference between revisions)
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<StructureSection load='6kks' size='340' side='right'caption='[[6kks]], [[Resolution|resolution]] 2.15Å' scene=''> | <StructureSection load='6kks' size='340' side='right'caption='[[6kks]], [[Resolution|resolution]] 2.15Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6kks]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KKS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6kks]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KKS FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WER ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kks OCA], [http://pdbe.org/6kks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kks RCSB], [http://www.ebi.ac.uk/pdbsum/6kks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kks ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kks OCA], [http://pdbe.org/6kks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kks RCSB], [http://www.ebi.ac.uk/pdbsum/6kks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kks ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/WER_ARATH WER_ARATH]] Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in roots and hypocotyl. Together with GL3 or BHLH2, promotes the formation of non-hair developing cells (atrichoblasts) et the N position in root epidermis. Regulates stomata spatial distribution in hypocotyls. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes such as GL2 and of CPC.<ref>PMID:10589676</ref> <ref>PMID:11585796</ref> <ref>PMID:14627722</ref> <ref>PMID:15361138</ref> <ref>PMID:15795220</ref> <ref>PMID:16207757</ref> | [[http://www.uniprot.org/uniprot/WER_ARATH WER_ARATH]] Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in roots and hypocotyl. Together with GL3 or BHLH2, promotes the formation of non-hair developing cells (atrichoblasts) et the N position in root epidermis. Regulates stomata spatial distribution in hypocotyls. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes such as GL2 and of CPC.<ref>PMID:10589676</ref> <ref>PMID:11585796</ref> <ref>PMID:14627722</ref> <ref>PMID:15361138</ref> <ref>PMID:15795220</ref> <ref>PMID:16207757</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | As the largest group of MYB family transcription factors, R2R3-MYB proteins play essential roles during plant growth and development. However, the structural basis underlying how R2R3-MYBs recognize the target DNA remains elusive. Here, we report the crystal structure of Arabidopsis WEREWOLF (WER), an R2R3-MYB protein, in complex with its target DNA. Structural analysis showed that the third alpha-helices in both the R2 and R3 repeats of WER fit in the major groove of the DNA, specifically recognizing the DNA motif 5'-AACNGC-3'. In combination with mutagenesis, in vitro binding and in vivo luciferase assays, we showed that K55, N106, K109 and N110 are critical for the function of WER. Although L59 of WER is not involved in DNA binding in the structure, ITC analysis suggested that L59 plays an important role in sensing DNA methylation at the fifth position of cytosine (5mC). Like 5mC, methylation at the sixth position of adenine (6mA) in the AAC element also inhibits the interaction between WER and its target DNA. Our study not only unravels the molecular basis of how WER recognizes its target DNA, but also suggests that 5mC and 6mA modifications may block the interaction between R2R3-MYB transcription factors and their target genes. | ||
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| + | Structural insights into target DNA recognition by R2R3-MYB transcription factors.,Wang B, Luo Q, Li Y, Yin L, Zhou N, Li X, Gan J, Dong A Nucleic Acids Res. 2019 Nov 16. pii: 5626523. doi: 10.1093/nar/gkz1081. PMID:31733060<ref>PMID:31733060</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6kks" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arath]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Luo, Q]] | [[Category: Luo, Q]] | ||
Revision as of 10:20, 4 December 2019
Structural insights into target DNA recognition by R2R3-type MYB transcription factor
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