6l3g
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases== | |
| + | <StructureSection load='6l3g' size='340' side='right'caption='[[6l3g]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l3g]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L3G FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3g OCA], [http://pdbe.org/6l3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3g RCSB], [http://www.ebi.ac.uk/pdbsum/6l3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pif1 plays multiple roles in maintaining genome stability and preferentially unwinds forked dsDNA, but the mechanism by which Pif1 unwinds forked dsDNA remains elusive. Here we report the structure of Bacteroides sp Pif1 (BaPif1) in complex with a symmetrical double forked dsDNA. Two interacting BaPif1 molecules are bound to each fork of the partially unwound dsDNA, and interact with the 5' arm and 3' ss/dsDNA respectively. Each of the two BaPif1 molecules is an active helicase and their interaction may regulate their helicase activities. The binding of BaPif1 to the 5' arm causes a sharp bend in the 5' ss/dsDNA junction, consequently breaking the first base-pair. BaPif1 bound to the 3' ss/dsDNA junction impacts duplex unwinding by stabilizing the unpaired first base-pair and engaging the second base-pair poised for breaking. Our results provide an unprecedented insight into how two BaPif1 coordinate with each other to unwind the forked dsDNA. | ||
| - | + | Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases.,Su N, Byrd AK, Bharath SR, Yang O, Jia Y, Tang X, Ha T, Raney KD, Song H Nat Commun. 2019 Nov 26;10(1):5375. doi: 10.1038/s41467-019-13414-9. PMID:31772234<ref>PMID:31772234</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6l3g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bharath, S R]] | ||
| + | [[Category: Song, H]] | ||
| + | [[Category: Su, N]] | ||
| + | [[Category: Atp dependent dna helicase]] | ||
| + | [[Category: Dsdna unwinding]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Hydrolase-dna complex]] | ||
| + | [[Category: Pif1]] | ||
Revision as of 15:06, 11 December 2019
Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases
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