| Structural highlights
Function
[RCSB_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981][1] [2] [3] [4] [5] [6] [7]
Publication Abstract from PubMed
RcsB is a highly conserved transcription regulator of the Rcs phosphorelay system, a complex two-component signal transduction system (N. Majdalani and S. Gottesman, Annu Rev Microbiol 59:379-405, 2005; A. J. Wolfe, Curr Opin Microbiol 13:204-209, 2010, https://doi.org/10.1016/j.mib.2010.01.002; D. J. Clarke, Future Microbiol 5:1173-1184, 2010, https://doi.org/10.2217/fmb.10.83). RcsB plays an important role in virulence and pathogenicity in human hosts by regulating biofilm formation. RcsB can regulate transcription alone or together with its auxiliary transcription regulators by forming heterodimers. This complexity allows RcsB to regulate transcription of more than 600 bacterial genes in response to different stresses (D. Wang et al., Mol Plant Microbe Interact 25:6-17, 2012, https://doi.org/10.1094/MPMI-08-11-0207). Despite increasing knowledge of RcsB importance, molecular mechanisms that drive the ability of RcsB to control transcription of a large number of genes remain unclear. Here, we present crystal structures of unphosphorylated RcsB in complex with the consensus DNA-binding sequence of 22-mer (DNA22) and 18-mer (DNA18) of the flhDC operon from Escherichia coli determined at 3.15- and 3.37-A resolution, respectively. The results of our structural analysis combined with the results of in vitro binding assays provide valuable insights to the protein regulatory mechanism, demonstrate how RcsB recognizes target DNA sequences, and reveal a unique oligomeric state that allows RcsB to form homo- and heterodimers. This information will help us understand the complex mechanisms of transcriptional regulation by RcsB in bacteria.IMPORTANCE RcsB is a well-studied two-component response regulator of the Rcs phosphorelay system, conserved within the family Enterobacteriaceae, which includes many pathogens. It is a global regulator, controlling more than 5% of bacterial genes associated with capsule biosynthesis, flagellar biogenesis, cell wall biosynthesis, antibiotic resistance, biofilm formation, and virulence in pathogens. Knowledge of RcsB structure represents a unique opportunity to explore mechanisms that regulate the Rcs phosphorelay system and its role in the family Enterobacteriaceae.
Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB.,Filippova EV, Zemaitaitis B, Aung T, Wolfe AJ, Anderson WF MBio. 2018 Feb 27;9(1). pii: mBio.01993-17. doi: 10.1128/mBio.01993-17. PMID:29487239[8]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wehland M, Bernhard F. The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria. J Biol Chem. 2000 Mar 10;275(10):7013-20. PMID:10702265
- ↑ Takeda S, Fujisawa Y, Matsubara M, Aiba H, Mizuno T. A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour. Mol Microbiol. 2001 Apr;40(2):440-50. PMID:11309126
- ↑ Davalos-Garcia M, Conter A, Toesca I, Gutierrez C, Cam K. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli. J Bacteriol. 2001 Oct;183(20):5870-6. PMID:11566985 doi:http://dx.doi.org/10.1128/JB.***********-5876.2001
- ↑ Hagiwara D, Sugiura M, Oshima T, Mori H, Aiba H, Yamashino T, Mizuno T. Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli. J Bacteriol. 2003 Oct;185(19):5735-46. PMID:13129944
- ↑ Gervais FG, Phoenix P, Drapeau GR. The rcsB gene, a positive regulator of colanic acid biosynthesis in Escherichia coli, is also an activator of ftsZ expression. J Bacteriol. 1992 Jun;174(12):3964-71. PMID:1597415
- ↑ Castanie-Cornet MP, Cam K, Bastiat B, Cros A, Bordes P, Gutierrez C. Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE. Nucleic Acids Res. 2010 Jun;38(11):3546-54. doi: 10.1093/nar/gkq097. Epub 2010, Feb 26. PMID:20189963 doi:http://dx.doi.org/10.1093/nar/gkq097
- ↑ Venkatesh GR, Kembou Koungni FC, Paukner A, Stratmann T, Blissenbach B, Schnetz K. BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS. J Bacteriol. 2010 Dec;192(24):6456-64. doi: 10.1128/JB.00807-10. Epub 2010 Oct, 15. PMID:20952573 doi:http://dx.doi.org/10.1128/JB.00807-10
- ↑ Filippova EV, Zemaitaitis B, Aung T, Wolfe AJ, Anderson WF. Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB. MBio. 2018 Feb 27;9(1). pii: mBio.01993-17. doi: 10.1128/mBio.01993-17. PMID:29487239 doi:http://dx.doi.org/10.1128/mBio.01993-17
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