7gsp

From Proteopedia

Revision as of 16:45, 5 November 2007

RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE

Overview

Here we present a time-resolved crystallographic analysis of the, hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1., The use of a slow substrate and fast crystallization methods made it, possible to perform the study with conventional data-collection, techniques. The results support the idea that the hydrolysis reaction, proceeds through a mechanism that is the inverse of the, transesterification reaction. In addition, the structures provide an, explanation for the differential behavior of RNase T1 towards exo- and, endo-cyclic thiophosphates.

About this Structure

7GSP is a Single protein structure of sequence from Aspergillus oryzae with PO4 and SGP as ligands. Active as Ribonuclease T(1), with EC number 3.1.27.3 Structure known Active Sites: BI1, BI2, CA2 and CAT. Full crystallographic information is available from OCA.

Reference

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218

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