| Structural highlights
Function
[HAT2_SCHPO] Regulatory subunit of the histone acetylase B (HAT-B) complex (By similarity). The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing (By similarity). Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:15369671, PubMed:24774534, PubMed:24789708, PubMed:25375240). Maintains the deacetylated state of histones specifically in the central core of the centromeres (PubMed:15369671).[UniProtKB:P39984][1] [2] [3] [4] [MIS19_SCHPO] Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708, PubMed:25375240). Links mis16 and mis18 to recruit CENP-A through interacting with non-sense-mediated mRNA decay (NMD) factors and the SWI/SNF complex (PubMed:24774534). Links also mis18 with the CCAN/mis6/ctf19 complex to promote CENP-A assembly (PubMed:24789708).[5] [6] [7]
Publication Abstract from PubMed
The Mis18 complex, composed of Mis16, Eic1, and Mis18 in fission yeast, selectively deposits the centromere-specific histone H3 variant, CENP-A(Cnp1), at centromeres. How the intact Mis18 holo-complex oligomerizes and how Mis16, a well-known ubiquitous histone H4 chaperone, plays a centromere-specific role in the Mis18 holo-complex, remain unclear. Here, we report the stoichiometry of the intact Mis18 holo-complex as (Mis16)2:(Eic1)2:(Mis18)4 using analytical ultracentrifugation. We further determine the crystal structure of Schizosaccharomyces pombe Mis16 in complex with the C-terminal portion of Eic1 (Eic1-CT). Notably, Mis16 accommodates Eic1-CT through the binding pocket normally occupied by histone H4, indicating that Eic1 and H4 compete for the same binding site, providing a mechanism for Mis16 to switch its binding partner from histone H4 to Eic1. Thus, our analyses not only determine the stoichiometry of the intact Mis18 holo-complex but also uncover the molecular mechanism by which Mis16 plays a centromere-specific role through Eic1 association.
Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A(Cnp1)-Specific Assembly Factor through Eic1 Interaction.,An S, Koldewey P, Chik J, Subramanian L, Cho US Structure. 2018 May 1. pii: S0969-2126(18)30137-0. doi:, 10.1016/j.str.2018.04.012. PMID:29804820[8]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell. 2004 Sep 17;118(6):715-29. PMID:15369671 doi:http://dx.doi.org/10.1016/j.cell.2004.09.002
- ↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
- ↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
- ↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
- ↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
- ↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
- ↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
- ↑ An S, Koldewey P, Chik J, Subramanian L, Cho US. Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A(Cnp1)-Specific Assembly Factor through Eic1 Interaction. Structure. 2018 May 1. pii: S0969-2126(18)30137-0. doi:, 10.1016/j.str.2018.04.012. PMID:29804820 doi:http://dx.doi.org/10.1016/j.str.2018.04.012
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