5yeu

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==Structural and mechanistic analyses reveal a unique Cas4-like protein in the mimivirus virophage resistance element system==
==Structural and mechanistic analyses reveal a unique Cas4-like protein in the mimivirus virophage resistance element system==
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<StructureSection load='5yeu' size='340' side='right' caption='[[5yeu]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='5yeu' size='340' side='right'caption='[[5yeu]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5yeu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YEU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5yeu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Apmv Apmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YEU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MIMI_R354 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212035 APMV])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yeu OCA], [http://pdbe.org/5yeu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yeu RCSB], [http://www.ebi.ac.uk/pdbsum/5yeu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yeu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yeu OCA], [http://pdbe.org/5yeu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yeu RCSB], [http://www.ebi.ac.uk/pdbsum/5yeu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yeu ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A clustered regularly interspaced short palindromic repeats (CRISPR)-like "mimivirus virophage resistance element" (MIMIVIRE) system, which contains specific cascade genes and a CRISPR array against virophages, was reported in mimiviruses. An essential component of the MIMIVIRE system is R354, encoding a nuclease and a likely functional homolog of Cas4. Here we show that R354 is a dual nuclease with both exonuclease and endonuclease activities. Structural analysis revealed that the catalytic core domain of R354 is similar to those of Cas4 and ? exonuclease despite their low sequence identity. R354 forms a homodimer that is important for its exonuclease but not endonuclease activity. Structural comparisons between the active and semi-active states of R354 demonstrated that an activation loop adjacent to the catalytic site is critical for enzymatic activity. Overall, the results suggest that R354 belongs to a novel MIMIVIRE system involved in innate virus immunity and provides a template for the identification of new CRISPR systems in other species.
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Structural and Mechanistic Analyses Reveal a Unique Cas4-like Protein in the Mimivirus Virophage Resistance Element System.,Dou C, Yu M, Gu Y, Wang J, Yin K, Nie C, Zhu X, Qi S, Wei Y, Cheng W iScience. 2018 May 25;3:1-10. doi: 10.1016/j.isci.2018.04.001. Epub 2018 Apr 11. PMID:30428313<ref>PMID:30428313</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5yeu" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Apmv]]
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[[Category: Large Structures]]
[[Category: Cheng, W]]
[[Category: Cheng, W]]
[[Category: Dou, C]]
[[Category: Dou, C]]

Revision as of 09:14, 18 December 2019

Structural and mechanistic analyses reveal a unique Cas4-like protein in the mimivirus virophage resistance element system

PDB ID 5yeu

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