6dtd
From Proteopedia
(Difference between revisions)
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==High-resolution crystal structure of Cas13b from Prevotella buccae== | ==High-resolution crystal structure of Cas13b from Prevotella buccae== | ||
| - | <StructureSection load='6dtd' size='340' side='right' caption='[[6dtd]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='6dtd' size='340' side='right'caption='[[6dtd]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6dtd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DTD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33574 Atcc 33574]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DTD FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF6485_0083 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28126 ATCC 33574])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dtd OCA], [http://pdbe.org/6dtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dtd RCSB], [http://www.ebi.ac.uk/pdbsum/6dtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dtd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dtd OCA], [http://pdbe.org/6dtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dtd RCSB], [http://www.ebi.ac.uk/pdbsum/6dtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dtd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members. Cas13b, which has been used for both RNA editing and nucleic acid detection, is unique among type VI CRISPR effectors in its linear domain architecture and CRISPR RNA (crRNA) structure. Here, we report the crystal structure of Prevotella buccae Cas13b (PbuCas13b) bound to crRNA at 1.65 A resolution. This structure, combined with biochemical experiments assaying the stability, kinetics, and function of Cas13b, provides a mechanistic model for Cas13b target RNA recognition and identifies features responsible for target and cleavage specificity. Based on these observations, we generated Cas13b variants with altered cleavage preferences, which may expand the utility of nuclease-based RNA detection assays and other applications of Cas13b in mammalian cells. | ||
| + | |||
| + | High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage.,Slaymaker IM, Mesa P, Kellner MJ, Kannan S, Brignole E, Koob J, Feliciano PR, Stella S, Abudayyeh OO, Gootenberg JS, Strecker J, Montoya G, Zhang F Cell Rep. 2019 Mar 26;26(13):3741-3751.e5. doi: 10.1016/j.celrep.2019.02.094. PMID:30917325<ref>PMID:30917325</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6dtd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 33574]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Slaymaker, I M.S]] | [[Category: Slaymaker, I M.S]] | ||
[[Category: Zhang, F Z]] | [[Category: Zhang, F Z]] | ||
[[Category: Hydrolase-rna complex]] | [[Category: Hydrolase-rna complex]] | ||
[[Category: Nuclease]] | [[Category: Nuclease]] | ||
Revision as of 10:14, 18 December 2019
High-resolution crystal structure of Cas13b from Prevotella buccae
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