6p1a
From Proteopedia
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<StructureSection load='6p1a' size='340' side='right'caption='[[6p1a]], [[Resolution|resolution]] 2.84Å' scene=''> | <StructureSection load='6p1a' size='340' side='right'caption='[[6p1a]], [[Resolution|resolution]] 2.84Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6p1a]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P1A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6p1a]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_21 Bacteriophage 21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P1A FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p1a OCA], [http://pdbe.org/6p1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p1a RCSB], [http://www.ebi.ac.uk/pdbsum/6p1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p1a ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p1a OCA], [http://pdbe.org/6p1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p1a RCSB], [http://www.ebi.ac.uk/pdbsum/6p1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p1a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding bacteriophage late genes. Q loads onto RNAP engaged in promoter-proximal pausing at a Q binding element (QBE) and adjacent sigma-dependent pause element (SDPE) to yield a Q-loading complex, and Q subsequently translocates with RNAP as a pausing-deficient, termination-deficient Q-loaded complex. Here, we report high-resolution structures of 4 states on the pathway of antitermination by Q from bacteriophage 21 (Q21): Q21, the Q21-QBE complex, the Q21-loading complex, and the Q21-loaded complex. The results show that Q21 forms a torus, a "nozzle," that narrows and extends the RNAP RNA-exit channel, extruding topologically linked single-stranded RNA and preventing the formation of pause and terminator hairpins. | ||
| + | |||
| + | Structural basis of Q-dependent antitermination.,Yin Z, Kaelber JT, Ebright RH Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18384-18390. doi:, 10.1073/pnas.1909801116. Epub 2019 Aug 27. PMID:31455742<ref>PMID:31455742</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6p1a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacteriophage 21]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ebright, R H]] | [[Category: Ebright, R H]] | ||
Revision as of 12:17, 18 December 2019
Transcription antitermination factor Q21 in complex with Q21-binding-element DNA
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