2v9p
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Concerted, stochastic and sequential mechanisms of action have been, proposed for different hexameric AAA+ molecular motors. Here we report the, crystal structure of the E1 helicase from bovine papillomavirus, where, asymmetric assembly is for the first time observed in the absence of, nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt, specific conformations linked to positional changes in the DNA-binding, hairpins, which follow a wave-like trajectory, as observed previously in, the E1/DNA/ADP complex. The protein's assembly thus maintains such an, asymmetric state in the absence of DNA and nucleotide cofactors, allowing, consideration of the E1 helicase action as the propagation of a, conformational wave around the protein ring. The data imply that the, wave's .. | + | Concerted, stochastic and sequential mechanisms of action have been, proposed for different hexameric AAA+ molecular motors. Here we report the, crystal structure of the E1 helicase from bovine papillomavirus, where, asymmetric assembly is for the first time observed in the absence of, nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt, specific conformations linked to positional changes in the DNA-binding, hairpins, which follow a wave-like trajectory, as observed previously in, the E1/DNA/ADP complex. The protein's assembly thus maintains such an, asymmetric state in the absence of DNA and nucleotide cofactors, allowing, consideration of the E1 helicase action as the propagation of a, conformational wave around the protein ring. The data imply that the, wave's propagation within the AAA+ domains is not necessarily coupled with, a strictly sequential hydrolysis of ATP. Since a single ATP hydrolysis, event would affect the whole hexamer, such events may simply serve to, rectify the direction of the wave's motion. |
==About this Structure== | ==About this Structure== | ||
| - | 2V9P is a | + | 2V9P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_2 Bovine papillomavirus type 2] with PO4 and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V9P OCA]. |
==Reference== | ==Reference== | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:53:53 2007'' |
Revision as of 16:48, 5 November 2007
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CRYSTAL STRUCTURE OF PAPILLOMAVIRUS E1 HEXAMERIC HELICASE DNA-FREE FORM
Overview
Concerted, stochastic and sequential mechanisms of action have been, proposed for different hexameric AAA+ molecular motors. Here we report the, crystal structure of the E1 helicase from bovine papillomavirus, where, asymmetric assembly is for the first time observed in the absence of, nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt, specific conformations linked to positional changes in the DNA-binding, hairpins, which follow a wave-like trajectory, as observed previously in, the E1/DNA/ADP complex. The protein's assembly thus maintains such an, asymmetric state in the absence of DNA and nucleotide cofactors, allowing, consideration of the E1 helicase action as the propagation of a, conformational wave around the protein ring. The data imply that the, wave's propagation within the AAA+ domains is not necessarily coupled with, a strictly sequential hydrolysis of ATP. Since a single ATP hydrolysis, event would affect the whole hexamer, such events may simply serve to, rectify the direction of the wave's motion.
About this Structure
2V9P is a Single protein structure of sequence from Bovine papillomavirus type 2 with PO4 and MG as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors., Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA, Nucleic Acids Res. 2007 Sep 19;. PMID:17881379
Page seeded by OCA on Mon Nov 5 18:53:53 2007
Categories: Bovine papillomavirus type 2 | Single protein | Antson, A.A. | Isupov, M.N. | Kovalevskiy, O.V. | Lebedev, A.A. | Sanders, C.M. | Sizov, D. | MG | PO4 | Aaa+ molecular motor | Atp-binding | Atpase | Dna replication | Dna translocation | Dna-binding | Early protein | Helicase | Hydrolase | Nucleotide-binding | Nucleus | Replication
