3w8r
From Proteopedia
(Difference between revisions)
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==Mutant structure of Thermus thermophilus HB8 uridine-cytidine kinase== | ==Mutant structure of Thermus thermophilus HB8 uridine-cytidine kinase== | ||
| - | <StructureSection load='3w8r' size='340' side='right' caption='[[3w8r]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3w8r' size='340' side='right'caption='[[3w8r]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3w8r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W8R FirstGlance]. <br> | <table><tr><td colspan='2'>[[3w8r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W8R FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w8r OCA], [http://pdbe.org/3w8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w8r RCSB], [http://www.ebi.ac.uk/pdbsum/3w8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w8r ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w8r OCA], [http://pdbe.org/3w8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w8r RCSB], [http://www.ebi.ac.uk/pdbsum/3w8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w8r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Uridine-cytidine kinase (UCK), including human UCK2, are a family of enzymes that generally phosphorylate both uridine and cytidine. However, UCK of Thermus thermophilus HB8 (ttCK) phosphorylates only cytidine. This cytidine-restricted activity is thought to depend on Tyr93, although the precise mechanism remains unresolved. Exhaustive mutagenesis of Tyr93 in ttCK revealed that the uridine phosphorylation activity was restored only by replacement of Tyr93 with His or Gln. Replacement of His117 in human UCK2, corresponding to residue Tyr93 in ttCK, by Tyr resulted in a loss of uridine phosphorylation activity. These findings indicated that uridine phosphorylation activity commonly depends on a single residue in the UCK family. | ||
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| + | Indispensable residue for uridine binding in the uridine-cytidine kinase family.,Tomoike F, Nakagawa N, Fukui K, Yano T, Kuramitsu S, Masui R Biochem Biophys Rep. 2017 Jul 8;11:93-98. doi: 10.1016/j.bbrep.2017.07.002., eCollection 2017 Sep. PMID:28955773<ref>PMID:28955773</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3w8r" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Journal:Acta Cryst F:S2053230X19002693|Journal:Acta Cryst F:S2053230X19002693]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Thet8]] | [[Category: Thet8]] | ||
[[Category: Uridine kinase]] | [[Category: Uridine kinase]] | ||
Revision as of 09:55, 25 December 2019
Mutant structure of Thermus thermophilus HB8 uridine-cytidine kinase
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Categories: Large Structures | Thet8 | Uridine kinase | Kuramitsu, S | Masui, R | Nakagawa, N | Tomoike, F | Kinase | Transferase
