3wef
From Proteopedia
(Difference between revisions)
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==Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate== | ==Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate== | ||
| - | <StructureSection load='3wef' size='340' side='right' caption='[[3wef]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution|resolution]] 2.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEF FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEF FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [http://pdbe.org/3wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [http://www.ebi.ac.uk/pdbsum/3wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [http://pdbe.org/3wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [http://www.ebi.ac.uk/pdbsum/3wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Squalene synthase (SQS) is a divalent metal-ion-dependent enzyme that catalyzes the two-step reductive `head-to-head' condensation of two molecules of farnesyl pyrophosphate to form squalene using presqualene diphosphate (PSPP) as an intermediate. In this paper, the structures of human SQS and its mutants in complex with several substrate analogues and intermediates coordinated with Mg2+ or Mn2+ are presented, which stepwise delineate the biosynthetic pathway. Extensive study of the SQS active site has identified several critical residues that are involved in binding reduced nicotinamide dinucleotide phosphate (NADPH). Based on mutagenesis data and a locally closed (JK loop-in) structure observed in the hSQS-(F288L)-PSPP complex, an NADPH-binding model is proposed for SQS. The results identified four major steps (substrate binding, condensation, intermediate formation and translocation) of the ordered sequential mechanisms involved in the `1'-1' isoprenoid biosynthetic pathway. These new findings clarify previous hypotheses based on site-directed mutagenesis and biochemical analysis. | ||
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| + | Structural insights into the catalytic mechanism of human squalene synthase.,Liu CI, Jeng WY, Chang WJ, Shih MF, Ko TP, Wang AH Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):231-41. doi:, 10.1107/S1399004713026230. Epub 2014 Jan 17. PMID:24531458<ref>PMID:24531458</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wef" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Squalene synthase|Squalene synthase]] | *[[Squalene synthase|Squalene synthase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Squalene synthase]] | [[Category: Squalene synthase]] | ||
[[Category: Jeng, W Y]] | [[Category: Jeng, W Y]] | ||
Revision as of 09:56, 25 December 2019
Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate
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