3wq8
From Proteopedia
(Difference between revisions)
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==Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form== | ==Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form== | ||
| - | <StructureSection load='3wq8' size='340' side='right' caption='[[3wq8]], [[Resolution|resolution]] 2.81Å' scene=''> | + | <StructureSection load='3wq8' size='340' side='right'caption='[[3wq8]], [[Resolution|resolution]] 2.81Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wq8]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQ8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wq8]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQ8 FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq8 OCA], [http://pdbe.org/3wq8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wq8 RCSB], [http://www.ebi.ac.uk/pdbsum/3wq8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq8 OCA], [http://pdbe.org/3wq8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wq8 RCSB], [http://www.ebi.ac.uk/pdbsum/3wq8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | One of the beta-glucosidases from Pyrococcus furiosus (BGLPf) is found to be a hyperthermophilic tetrameric enzyme that can degrade cellooligosaccharides. Recently, the crystal structures of the tetrameric and dimeric forms were solved. Here, a new monomeric form of BGLPf was constructed by removing the C-terminal region of the enzyme and its crystal structure was solved at a resolution of 2.8 A in space group P1. It was discovered that the mutant enzyme forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal. Under biological conditions, the mutant enzyme forms a monomer. This result helps explain how BGLPf has attained its oligomeric structure and thermostability. | ||
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| + | Monomer structure of a hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form.,Nakabayashi M, Kataoka M, Watanabe M, Ishikawa K Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):854-9. doi:, 10.1107/S2053230X14010188. Epub 2014 Jun 18. PMID:25005077<ref>PMID:25005077</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wq8" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Beta-glucosidase|Beta-glucosidase]] | + | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] |
| - | *[[Galactosidase|Galactosidase]] | + | *[[Galactosidase 3D structures|Galactosidase 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 43587]] | [[Category: Atcc 43587]] | ||
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Ishikawa, K]] | [[Category: Ishikawa, K]] | ||
[[Category: Kataoka, M]] | [[Category: Kataoka, M]] | ||
Revision as of 09:57, 25 December 2019
Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form
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