3wqj
From Proteopedia
(Difference between revisions)
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==Crystal structure of archaerhodopsin-2 at 1.8 angstrom resolution== | ==Crystal structure of archaerhodopsin-2 at 1.8 angstrom resolution== | ||
| - | <StructureSection load='3wqj' size='340' side='right' caption='[[3wqj]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3wqj' size='340' side='right'caption='[[3wqj]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium Halobacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQJ FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium Halobacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQJ FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqj OCA], [http://pdbe.org/3wqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wqj RCSB], [http://www.ebi.ac.uk/pdbsum/3wqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqj ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqj OCA], [http://pdbe.org/3wqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wqj RCSB], [http://www.ebi.ac.uk/pdbsum/3wqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Archaerhodopsin-2 (aR2), the sole protein found in the claret membrane of Halorubrum sp. Aus-2, functions as a light-driven proton pump. In this study, structural analysis of aR2 was performed using a novel three-dimensional crystal prepared by the successive fusion of claret membranes. The crystal is made up of stacked membranes, in each of which aR2 trimers are arranged on a hexagonal lattice. This lattice structure resembles that found in the purple membrane of H. salinarum, except that lipid molecules trapped within the trimeric structure are not distributed with perfect threefold symmetry. Nonetheless, diffraction data at 1.8 A resolution provide accurate structural information about functionally important residues. It is shown that two glutamates in the proton-release channel form a paired structure that is maintained by a low-barrier hydrogen bond. Although the structure of the proton-release pathway is highly conserved among proton-pumping archaeal rhodopsins, aR2 possesses the following peculiar structural features: (i) the motional freedom of the tryptophan residue that makes contact with the C13 methyl group of retinal is restricted, affecting the formation/decay kinetics of the L state, and (ii) the N-terminal polypeptide folds into an Omega-loop, which may play a role in organizing the higher-order structure. | ||
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| + | Structure of archaerhodopsin-2 at 1.8 A resolution.,Kouyama T, Fujii R, Kanada S, Nakanishi T, Chan SK, Murakami M Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2692-701. doi:, 10.1107/S1399004714017313. Epub 2014 Sep 27. PMID:25286853<ref>PMID:25286853</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wqj" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Rhodopsin|Rhodopsin]] | + | *[[Rhodopsin 3D structures|Rhodopsin 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Halobacterium]] | [[Category: Halobacterium]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Kouyama, T]] | [[Category: Kouyama, T]] | ||
[[Category: Trans-membrane helice]] | [[Category: Trans-membrane helice]] | ||
[[Category: Light-driven proton pump]] | [[Category: Light-driven proton pump]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 08:36, 1 January 2020
Crystal structure of archaerhodopsin-2 at 1.8 angstrom resolution
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