3wkv
From Proteopedia
(Difference between revisions)
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==Voltage-gated proton channel: VSOP/Hv1 chimeric channel== | ==Voltage-gated proton channel: VSOP/Hv1 chimeric channel== | ||
| - | <StructureSection load='3wkv' size='340' side='right' caption='[[3wkv]], [[Resolution|resolution]] 3.45Å' scene=''> | + | <StructureSection load='3wkv' size='340' side='right'caption='[[3wkv]], [[Resolution|resolution]] 3.45Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wkv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WKV FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wkv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WKV FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wkv OCA], [http://pdbe.org/3wkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wkv RCSB], [http://www.ebi.ac.uk/pdbsum/3wkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wkv ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wkv OCA], [http://pdbe.org/3wkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wkv RCSB], [http://www.ebi.ac.uk/pdbsum/3wkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wkv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The voltage-gated proton channel Hv1 (or VSOP) has a voltage-sensor domain (VSD) with dual roles of voltage sensing and proton permeation. Its gating is sensitive to pH and Zn(2+). Here we present a crystal structure of mouse Hv1 in the resting state at 3.45-A resolution. The structure showed a 'closed umbrella' shape with a long helix consisting of the cytoplasmic coiled coil and the voltage-sensing helix, S4, and featured a wide inner-accessible vestibule. Two out of three arginines in S4 were located below the phenylalanine constituting the gating charge-transfer center. The extracellular region of each protomer coordinated a Zn(2+), thus suggesting that Zn(2+) stabilizes the resting state of Hv1 by competing for acidic residues that otherwise form salt bridges with voltage-sensing positive charges on S4. These findings provide a platform for understanding the general principles of voltage sensing and proton permeation. | ||
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| + | X-ray crystal structure of voltage-gated proton channel.,Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, Okochi Y, Matsuda M, Narita H, Okamura Y, Nakagawa A Nat Struct Mol Biol. 2014 Apr;21(4):352-7. doi: 10.1038/nsmb.2783. Epub 2014 Mar , 2. PMID:24584463<ref>PMID:24584463</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wkv" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Ion channels|Ion channels]] | + | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Lk3 transgenic mice]] | [[Category: Lk3 transgenic mice]] | ||
[[Category: Fujiwara, Y]] | [[Category: Fujiwara, Y]] | ||
Revision as of 08:36, 1 January 2020
Voltage-gated proton channel: VSOP/Hv1 chimeric channel
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