4m0l
From Proteopedia
(Difference between revisions)
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==Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus complexed with GDP== | ==Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus complexed with GDP== | ||
| - | <StructureSection load='4m0l' size='340' side='right' caption='[[4m0l]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4m0l' size='340' side='right'caption='[[4m0l]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4m0l]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[4m0l]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3p3m 3p3m]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M0L FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m2l|4m2l]], [[4m4s|4m4s]], [[4m53|4m53]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m2l|4m2l]], [[4m4s|4m4s]], [[4m53|4m53]]</td></tr> | ||
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eif2g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eif2g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m0l OCA], [http://pdbe.org/4m0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m0l RCSB], [http://www.ebi.ac.uk/pdbsum/4m0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m0l ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m0l OCA], [http://pdbe.org/4m0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m0l RCSB], [http://www.ebi.ac.uk/pdbsum/4m0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m0l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/IF2G_SULSO IF2G_SULSO]] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). | [[http://www.uniprot.org/uniprot/IF2G_SULSO IF2G_SULSO]] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In eukaryotes and archaea, the heterotrimeric translation initiation factor 2 (e/aIF2) is pivotal for the delivery of methionylated initiator tRNA (Met-tRNA(i)) to the ribosome. It acts as a molecular switch that cycles between inactive (GDP-bound) and active (GTP-bound) states. Recent studies show that eIF2 can also exist in a long-lived eIF2gamma-GDP-P(i) (inorganic phosphate) active state. Here, four high-resolution crystal structures of aIF2gamma from Sulfolobus solfataricus are reported: aIF2gamma-GDPCP (a nonhydrolyzable GTP analogue), aIF2gamma-GDP-formate (in which a formate ion possibly mimics P(i)), aIF2gamma-GDP and nucleotide-free aIF2gamma. The structures describe the different states of aIF2gamma and demonstrate the conformational transitions that take place in the aIF2gamma `life cycle'. | ||
| + | |||
| + | Conformational transitions in the gamma subunit of the archaeal translation initiation factor 2.,Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):658-67. doi:, 10.1107/S1399004713032240. Epub 2014 Feb 15. PMID:24598735<ref>PMID:24598735</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4m0l" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Sacs2]] | ||
[[Category: Arkhipova, V I]] | [[Category: Arkhipova, V I]] | ||
[[Category: Gabdulkhakov, A G]] | [[Category: Gabdulkhakov, A G]] | ||
Revision as of 08:38, 1 January 2020
Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus complexed with GDP
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Categories: Large Structures | Sacs2 | Arkhipova, V I | Gabdulkhakov, A G | Garber, M B | Lazopulo, A M | Lazopulo, S M | Nikonov, O S | Nikonov, S V | Nikulin, A D | Stolboushkina, E A | Aif2 | Aif2 gamma subunit | G-protein | Gtp-binding | Nucleotide-binding | Protein biosynthesis | Rna-binding | Translation | Translation initiation
