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6b3x

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Revision as of 10:12, 1 January 2020

Crystal structure of CstF-50 in complex with CstF-77

Structural highlights

6b3x is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CSTF1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CSTF1_HUMAN] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. [CSTF3_HUMAN] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.

Publication Abstract from PubMed

Cleavage stimulation factor (CstF) is a highly conserved protein complex composed of three subunits that recognizes G/U-rich sequences downstream of the polyadenylation signal of eukaryotic mRNAs. While CstF has been identified over 25 years ago, the architecture and contribution of each subunit to RNA recognition have not been fully understood. In this study, we provide a structural basis for the recruitment of CstF-50 to CstF via interaction with CstF-77 and establish that the hexameric assembly of CstF creates a high affinity platform to target various G/U-rich sequences. We further demonstrate that CstF-77 boosts the affinity of the CstF-64 RRM to the RNA targets and CstF-50 fine tunes the ability of the complex to recognize G/U sequences of certain lengths and content.

Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals.,Yang W, Hsu PL, Yang F, Song JE, Varani G Nucleic Acids Res. 2017 Nov 24. pii: 4647674. doi: 10.1093/nar/gkx1177. PMID:29186539^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang W, Hsu PL, Yang F, Song JE, Varani G. Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals. Nucleic Acids Res. 2017 Nov 24. pii: 4647674. doi: 10.1093/nar/gkx1177. PMID:29186539 doi:http://dx.doi.org/10.1093/nar/gkx1177

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b3x"

@@ Line 1: / Line 1: @@
 ==Crystal structure of CstF-50 in complex with CstF-77==
-<StructureSection load='6b3x' size='340' side='right' caption='[[6b3x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='6b3x' size='340' side='right'caption='[[6b3x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6b3x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B3X FirstGlance]. <br>
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Hsu, P]]
 [[Category: Song, J E]]

6b3x

From Proteopedia

Revision as of 10:12, 1 January 2020

Crystal structure of CstF-50 in complex with CstF-77

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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