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6bw0

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Revision as of 10:28, 1 January 2020

Nipah virus W protein C-terminus in complex with Importin alpha 1

Structural highlights

6bw0 is a 2 chain structure with sequence from Lk3 transgenic mice and Nipav. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6bvt, 6bvz, 6bvv
Gene:	P/V/C (NIPAV), Kpna2, Rch1 (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[W_NIPAV] Prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta). Interacts with host STAT1 protein in the nucleus, blocking it's phosphorylation by IFN-alpha/beta. Also blocks antiviral state induced by Toll-like receptor 3/TLR3 binding to dsRNA. [IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Publication Abstract from PubMed

Seven human isoforms of importin alpha mediate nuclear import of cargo in a tissue- and isoform-specific manner. How nuclear import adaptors differentially interact with cargo harbouring the same nuclear localisation signal (NLS) remains poorly understood, as the NLS recognition region is highly conserved. Here, we provide a structural basis for the nuclear import specificity of W proteins in Hendra and Nipah viruses. We determine the structural interfaces of these cargo bound to importin alpha1 and alpha3, identifying a 2.4-fold more extensive interface and > 50-fold higher binding affinity for importin alpha3. Through the design of importin alpha1 and alpha3 chimeric and mutant proteins, together with structures of cargo-free importin alpha1 and alpha3 isoforms, we establish that the molecular basis of specificity resides in the differential positioning of the armadillo repeats 7 and 8. Overall, our study provides mechanistic insights into a range of important nucleocytoplasmic transport processes reliant on isoform adaptor specificity.

Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses.,Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragao D, Basler CF, Forwood JK Nat Commun. 2018 Sep 12;9(1):3703. doi: 10.1038/s41467-018-05928-5. PMID:30209309^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragao D, Basler CF, Forwood JK. Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses. Nat Commun. 2018 Sep 12;9(1):3703. doi: 10.1038/s41467-018-05928-5. PMID:30209309 doi:http://dx.doi.org/10.1038/s41467-018-05928-5

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6bw0"

@@ Line 1: / Line 1: @@
 ==Nipah virus W protein C-terminus in complex with Importin alpha 1==
-<StructureSection load='6bw0' size='340' side='right' caption='[[6bw0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='6bw0' size='340' side='right'caption='[[6bw0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6bw0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Nipav Nipav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BW0 FirstGlance]. <br>
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Importin|Importin]]
+*[[Importin 3D structures|Importin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Lk3 transgenic mice]]
 [[Category: Nipav]]

6bw0

From Proteopedia

Revision as of 10:28, 1 January 2020

Nipah virus W protein C-terminus in complex with Importin alpha 1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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