1a8f
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1a8f.gif|left|200px]] | [[Image:1a8f.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1a8f", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1a8f| PDB=1a8f | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE''' | '''HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE''' | ||
| Line 39: | Line 36: | ||
[[Category: Wang, Y.]] | [[Category: Wang, Y.]] | ||
[[Category: Woodworth, R C.]] | [[Category: Woodworth, R C.]] | ||
| - | [[Category: | + | [[Category: Carbonate]] |
| - | [[Category: | + | [[Category: Glycoprotein]] |
| - | [[Category: | + | [[Category: Iron transport]] |
| - | [[Category: | + | [[Category: Iron-release]] |
| - | [[Category: | + | [[Category: Nlobe]] |
| - | [[Category: | + | [[Category: Transferrin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:58:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:58, 2 May 2008
HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE
Overview
The N-lobe of human serum transferrin (hTF/2N) has been expressed in baby hamster kidney cells and crystallized in both orthorhombic (P212121) and tetragonal (P41212) space groups. Both crystal forms diffract to high resolution (1.6 and 1.8 A, respectively) and have been solved by molecular replacement. Subsequent refinement resulted in final models for the structure of hTF/2N that had crystallographic R-factors of 18.1 and 19.7% for the two crystal forms, respectively; these models represent the highest-resolution transferrin structures determined to date. The hTF/2N polypeptide has a folding pattern similar to those of other transferrins, including the presence of a deep cleft that contains the metal-binding site. In contrast to other transferrins, both crystal forms of hTF/2N display disorder at the iron-binding site; model building suggests that this disorder consists of alternative conformations of the synergistically bound carbonate anion, the side chain for Arg-124, and several solvent molecules. Subsequent refinement revealed that conformation A has an occupancy of 0.63-0. 65 and corresponds to the structure of the iron-binding site found in other transferrins. The alternative conformation B has an occupancy of 0.35-0.37; in this structure, the carbonate has rotated 30 degrees relative to the iron and the side chain for Arg-124 has moved to accommodate the new carbonate position. Several water molecules appear to stabilize the carbonate anion in the two conformations. These structures are consistent with the protonation of the carbonate and resulting partial removal of the anion from the metal; these events would occur prior to cleft opening and metal release.
About this Structure
1A8F is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Two high-resolution crystal structures of the recombinant N-lobe of human transferrin reveal a structural change implicated in iron release., MacGillivray RT, Moore SA, Chen J, Anderson BF, Baker H, Luo Y, Bewley M, Smith CA, Murphy ME, Wang Y, Mason AB, Woodworth RC, Brayer GD, Baker EN, Biochemistry. 1998 Jun 2;37(22):7919-28. PMID:9609685 Page seeded by OCA on Fri May 2 09:58:07 2008
Categories: Homo sapiens | Single protein | Anderson, B F. | Baker, E N. | Baker, H. | Bewley, M. | Brayer, G D. | Chen, J. | Luo, Y. | Macgillivray, R T.A. | Mason, A B. | Moore, S A. | Murphy, M E.P. | Smith, C A. | Wang, Y. | Woodworth, R C. | Carbonate | Glycoprotein | Iron transport | Iron-release | Nlobe | Transferrin
