6jpu
From Proteopedia
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<StructureSection load='6jpu' size='340' side='right'caption='[[6jpu]], [[Resolution|resolution]] 4.27Å' scene=''> | <StructureSection load='6jpu' size='340' side='right'caption='[[6jpu]], [[Resolution|resolution]] 4.27Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6jpu]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JPU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jpu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JPU FirstGlance]. <br> |
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6jpq|6jpq]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6jpq|6jpq]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPAC31G5.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jpu OCA], [http://pdbe.org/6jpu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jpu RCSB], [http://www.ebi.ac.uk/pdbsum/6jpu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jpu ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jpu OCA], [http://pdbe.org/6jpu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jpu RCSB], [http://www.ebi.ac.uk/pdbsum/6jpu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jpu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The fundamental unit of chromatin, the nucleosome, is an intricate structure that requires histone chaperones for assembly. ATAD2 AAA+ ATPases are a family of histone chaperones that regulate nucleosome density and chromatin dynamics. Here, we demonstrate that the fission yeast ATAD2 homolog, Abo1, deposits histone H3-H4 onto DNA in an ATP-hydrolysis-dependent manner by in vitro reconstitution and single-tethered DNA curtain assays. We present cryo-EM structures of an ATAD2 family ATPase to atomic resolution in three different nucleotide states, revealing unique structural features required for histone loading on DNA, and directly visualize the transitions of Abo1 from an asymmetric spiral (ATP-state) to a symmetric ring (ADP- and apo-states) using high-speed atomic force microscopy (HS-AFM). Furthermore, we find that the acidic pore of ATP-Abo1 binds a peptide substrate which is suggestive of a histone tail. Based on these results, we propose a model whereby Abo1 facilitates H3-H4 loading by utilizing ATP. | ||
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| + | Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.,Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ Nat Commun. 2019 Dec 17;10(1):5764. doi: 10.1038/s41467-019-13743-9. PMID:31848341<ref>PMID:31848341</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jpu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Fission yeast]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cho, C]] | [[Category: Cho, C]] | ||
Revision as of 11:18, 1 January 2020
CryoEM structure of Abo1 hexamer - apo complex
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