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6mpz
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a double glycine motif protease from AMS/PCAT transporter in complex with the leader peptide== | |
| + | <StructureSection load='6mpz' size='340' side='right'caption='[[6mpz]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mpz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_c6a11 Lachnospiraceae bacterium c6a11] and [http://en.wikipedia.org/wiki/Promm Promm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MPZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=16P:3,6,9,12,15,18-HEXAOXAICOSANE'>16P</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLZ:AMINO-ACETALDEHYDE'>GLZ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpz OCA], [http://pdbe.org/6mpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mpz RCSB], [http://www.ebi.ac.uk/pdbsum/6mpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The secretion of peptides and proteins is essential for survival and ecological adaptation of bacteria. Dual-functional ATP-binding cassette transporters export antimicrobial or quorum signaling peptides in Gram-positive bacteria. Their substrates contain a leader sequence that is excised by an N-terminal peptidase C39 domain at a double Gly motif. We characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can remove the leader peptide from a diverse set of peptides. The 2.0 A resolution crystal structure of the protease domain in complex with a covalently bound leader peptide demonstrates the basis for substrate recognition across the entire class of such transporters. The structural data also provide a model for understanding the role of leader peptide recognition in the translocation cycle, and the function of degenerate, non-functional C39-like domains (CLD) in substrate recruitment in toxin exporters in Gram-negative bacteria. | ||
| - | + | Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease.,Bobeica SC, Dong SH, Huo L, Mazo N, McLaughlin MI, Jimenez-Oses G, Nair SK, van der Donk WA Elife. 2019 Jan 14;8. pii: 42305. doi: 10.7554/eLife.42305. PMID:30638446<ref>PMID:30638446</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6mpz" style="background-color:#fffaf0;"></div> |
| - | [[Category: Dong, S | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Lachnospiraceae bacterium c6a11]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Promm]] | ||
| + | [[Category: Dong, S H]] | ||
| + | [[Category: Nair, S K]] | ||
| + | [[Category: Lantibiotic]] | ||
| + | [[Category: Leader peptide]] | ||
| + | [[Category: Peptidase c39 domain]] | ||
| + | [[Category: Peptide secretion]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 11:27, 1 January 2020
Crystal structure of a double glycine motif protease from AMS/PCAT transporter in complex with the leader peptide
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