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6r8i

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Revision as of 12:14, 1 January 2020

PP4R3A EVH1 domain bound to FxxP motif

Structural highlights

6r8i is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	PPP4R3A, KIAA2010, PP4R3A, SMEK1, MSTP033 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[P4R3A_HUMAN] Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.^[1]

Publication Abstract from PubMed

Dynamic protein phosphorylation constitutes a fundamental regulatory mechanism in all organisms. Phosphoprotein phosphatase 4 (PP4) is a conserved and essential nuclear serine and threonine phosphatase. Despite the importance of PP4, general principles of substrate selection are unknown, hampering the study of signal regulation by this phosphatase. Here, we identify and thoroughly characterize a general PP4 consensus-binding motif, the FxxP motif. X-ray crystallography studies reveal that FxxP motifs bind to a conserved pocket in the PP4 regulatory subunit PPP4R3. Systems-wide in silico searches integrated with proteomic analysis of PP4 interacting proteins allow us to identify numerous FxxP motifs in proteins controlling a range of fundamental cellular processes. We identify an FxxP motif in the cohesin release factor WAPL and show that this regulates WAPL phosphorylation status and is required for efficient cohesin release. Collectively our work uncovers basic principles of PP4 specificity with broad implications for understanding phosphorylation-mediated signaling in cells.

A Consensus Binding Motif for the PP4 Protein Phosphatase.,Ueki Y, Kruse T, Weisser MB, Sundell GN, Larsen MSY, Mendez BL, Jenkins NP, Garvanska DH, Cressey L, Zhang G, Davey N, Montoya G, Ivarsson Y, Kettenbach AN, Nilsson J Mol Cell. 2019 Sep 25. pii: S1097-2765(19)30684-7. doi:, 10.1016/j.molcel.2019.08.029. PMID:31585692^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chowdhury D, Xu X, Zhong X, Ahmed F, Zhong J, Liao J, Dykxhoorn DM, Weinstock DM, Pfeifer GP, Lieberman J. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. Mol Cell. 2008 Jul 11;31(1):33-46. doi: 10.1016/j.molcel.2008.05.016. PMID:18614045 doi:http://dx.doi.org/10.1016/j.molcel.2008.05.016
↑ Ueki Y, Kruse T, Weisser MB, Sundell GN, Larsen MSY, Mendez BL, Jenkins NP, Garvanska DH, Cressey L, Zhang G, Davey N, Montoya G, Ivarsson Y, Kettenbach AN, Nilsson J. A Consensus Binding Motif for the PP4 Protein Phosphatase. Mol Cell. 2019 Sep 25. pii: S1097-2765(19)30684-7. doi:, 10.1016/j.molcel.2019.08.029. PMID:31585692 doi:http://dx.doi.org/10.1016/j.molcel.2019.08.029

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6r8i"

@@ Line 3: / Line 3: @@
 <StructureSection load='6r8i' size='340' side='right'caption='[[6r8i]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6r8i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R8I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6r8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R8I FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r8i OCA], [http://pdbe.org/6r8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r8i RCSB], [http://www.ebi.ac.uk/pdbsum/6r8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r8i ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PPP4R3A, KIAA2010, PP4R3A, SMEK1, MSTP033 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r8i OCA], [http://pdbe.org/6r8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r8i RCSB], [http://www.ebi.ac.uk/pdbsum/6r8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r8i ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Large Structures]]
 [[Category: Cressey, L]]

6r8i

From Proteopedia

Revision as of 12:14, 1 January 2020

PP4R3A EVH1 domain bound to FxxP motif

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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