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5k94

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Revision as of 08:08, 8 January 2020

Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase

Structural highlights

5k94 is a 2 chain structure with sequence from Eco57. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	malE, Z5632, ECs5017 (ECO57)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MALE_ECO57] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).

Publication Abstract from PubMed

We coupled peptides from a CNBr digest of signal-sequenceless maltose-binding protein (MBP) to a surface plasmon resonance chip. SecA-N95, SecA-N68, and SecA-DM (which consists of only the DEAD Motor domains NBD1 and NBD2) bound to the immobilized peptides; ADP weakened the binding. SecA-DM, which lacks the 'preprotein cross-linking domain' (PPXD), displayed the most extensive binding, while an MBP-PPXD chimera showed no binding, demonstrating that the PPXD does not contribute to the binding. We characterized the sequence specificity using oriented peptide libraries; these results enabled synthesis of a 20-residue peptide that was used to recapitulate the results obtained with MBP-derived peptides. This study shows that there is a promiscuous and nucleotide-modulated peptide-binding site in the DEAD Motor domains of SecA.

Characterization of a polypeptide-binding site in the DEAD Motor of the SecA ATPase.,Khalili Yazdi A, Namjoshi S, Hackett J, Ghonaim N, Shilton BH FEBS Lett. 2017 Oct;591(20):3378-3390. doi: 10.1002/1873-3468.12832. Epub 2017, Sep 12. PMID:28862749^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Khalili Yazdi A, Namjoshi S, Hackett J, Ghonaim N, Shilton BH. Characterization of a polypeptide-binding site in the DEAD Motor of the SecA ATPase. FEBS Lett. 2017 Oct;591(20):3378-3390. doi: 10.1002/1873-3468.12832. Epub 2017, Sep 12. PMID:28862749 doi:http://dx.doi.org/10.1002/1873-3468.12832

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5k94"

@@ Line 1: / Line 1: @@
 ==Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase==
-<StructureSection load='5k94' size='340' side='right' caption='[[5k94]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='5k94' size='340' side='right'caption='[[5k94]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K94 FirstGlance]. <br>
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Eco57]]
+[[Category: Large Structures]]
 [[Category: Ghonaim, N]]
 [[Category: Hackett, J]]

5k94

From Proteopedia

Revision as of 08:08, 8 January 2020

Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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