6n1p

From Proteopedia

(Difference between revisions)

Revision as of 09:08, 8 January 2020

Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry

Structural highlights

6n1p is a 10 chain structure with sequence from Strp4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6n1q, 6n1r
Gene:	gyrA_1, gyrA, ERS409327_01712 (STRP4)
Activity:	DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A0A0Y2BJX7_STREE] A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.[HAMAP-Rule:MF_01897]

Publication Abstract from PubMed

Gyrase is a unique type IIA topoisomerase that uses ATP hydrolysis to maintain the negatively supercoiled state of bacterial DNA. In order to perform its function, gyrase undergoes a sequence of conformational changes that consist of concerted gate openings, DNA cleavage, and DNA strand passage events. Structures where the transported DNA molecule (T-segment) is trapped by the A subunit have not been observed. Here we present the cryoEM structures of two oligomeric complexes of open gyrase A dimers and DNA. The protein subunits in these complexes were solved to 4 A and 5.16 A resolution. One of the complexes traps a linear DNA molecule, a putative T-segment, which interacts with the open gyrase A dimers in two states, representing steps either prior to or after passage through the DNA-gate. The structures locate the T-segment in important intermediate conformations of the catalytic cycle and provide insights into gyrase-DNA interactions and mechanism.

CryoEM structures of open dimers of Gyrase A in complex with DNA illuminate mechanism of strand passage.,Soczek KM, Grant T, Rosenthal PB, Mondragon A Elife. 2018 Nov 20;7. pii: 41215. doi: 10.7554/eLife.41215. PMID:30457554^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Soczek KM, Grant T, Rosenthal PB, Mondragon A. CryoEM structures of open dimers of Gyrase A in complex with DNA illuminate mechanism of strand passage. Elife. 2018 Nov 20;7. pii: 41215. doi: 10.7554/eLife.41215. PMID:30457554 doi:http://dx.doi.org/10.7554/eLife.41215

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6n1p"

@@ Line 1: / Line 1: @@
 ==Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry==
-<StructureSection load='6n1p' size='340' side='right' caption='[[6n1p]], [[Resolution|resolution]] 6.35&Aring;' scene=''>
+<StructureSection load='6n1p' size='340' side='right'caption='[[6n1p]], [[Resolution|resolution]] 6.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6n1p]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6n1p]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Strp4 Strp4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1P FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6n1q|6n1q]], [[6n1r|6n1r]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gyrA_1, gyrA, ERS409327_01712 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=512566 STRP4])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1p OCA], [http://pdbe.org/6n1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1p RCSB], [http://www.ebi.ac.uk/pdbsum/6n1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1p ProSAT]</span></td></tr>
@@ Line 19: / Line 20: @@
 </div>
 <div class="pdbe-citations 6n1p" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Gyrase 3D Structures|Gyrase 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Strp4]]
 [[Category: Grant, T]]
 [[Category: Mondragon, A]]

6n1p

From Proteopedia

Revision as of 09:08, 8 January 2020

Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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