Sandbox Reserved 1094

From Proteopedia

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It also has a role in protecting cells from destruction because it produces a cofactor NADPH which plays a role in protecting cells from reactive oxygen species.
It also has a role in protecting cells from destruction because it produces a cofactor NADPH which plays a role in protecting cells from reactive oxygen species.
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== Disease ==
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== Genomic context ==
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It is coded by the G6PD gene (1461 nucleotides).
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== Relevance ==
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== Catalytic activity ==
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D-glucose 6-phosphate + NAD+ → 6-phospho-D-glucono-1,5-lactone + H+ + NADH
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KM=114 µM for G6PD (with NADP), KM=69 µM for G6PD (with NAD),
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KM=8.0 µM for NADP, KM=160 µM for NAD
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Its regulation depends on the concentration of substrate and coenzyme, rate limiting step in pentose phosphate pathway.
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== Optimal activity conditions ==
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Optimum pH is 5.4 - 8.9.
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== Evolutionary conservation ==
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We will keep the interactivity that exist on the page: a box can be checked to observe the different structures conserved evolutionary.
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== Mutations ==
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Mutagenesis inducing catalytic activity loss: +200 mutations have been identified. A change of amino acids leads to disruption of the normal
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structure/function/reduce the expression of enzymes.
== Structural highlights ==
== Structural highlights ==
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It is formed of a homodimer (dimer of two identical monomers).
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Depending on several conditions, it can dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP+/NADPH using the Rossman fold.
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

Revision as of 15:03, 8 January 2020

This Sandbox is Reserved from 25/11/2019, through 30/9/2020 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1091 through Sandbox Reserved 1115.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

Glucose-6-Phosphate Dehydrogenase from Leuconostoc mesenteroides

Caption for this structure

Drag the structure with the mouse to rotate

References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
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