Sandbox dav 4
From Proteopedia
(→Exploring the Structure) |
|||
| Line 7: | Line 7: | ||
, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The <scene name='Sandbox_dav_4/Gfp/1'>chromophore</scene> | , responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The <scene name='Sandbox_dav_4/Gfp/1'>chromophore</scene> | ||
forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or [[Ser65]])-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues'' | forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or [[Ser65]])-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues'' | ||
| + | == Mechanism == | ||
| + | '''dddd''' | ||
| + | == Related 3D structures == | ||
| + | ''ddddd'' deee dedee dede [[google.com/]] | ||
| + | <Structure load='6OYH' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | ||
Revision as of 23:25, 14 January 2020
Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria (PDB entry 1ema), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
Exploring the Structure
|
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The , responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues
Mechanism
dddd
Related 3D structures
ddddd deee dedee dede google.com/
|
