We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
6l9s
From Proteopedia
(Difference between revisions)
m (Protected "6l9s" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Na-dithionite reduced auracyanin from photosynthetic bacterium Roseiflexus castenholzii== | |
| + | <StructureSection load='6l9s' size='340' side='right'caption='[[6l9s]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l9s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Roseiflexus_castenholzii_(strain_dsm_13941_/_hlo8) Roseiflexus castenholzii (strain dsm 13941 / hlo8)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L9S FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6kol|6kol]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l9s OCA], [http://pdbe.org/6l9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l9s RCSB], [http://www.ebi.ac.uk/pdbsum/6l9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l9s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Auracyanin (Ac) is a blue copper protein that mediates the electron transfer between Alternative Complex III (ACIII) and downstream electron acceptors in both fort chains of filamentous anoxygenic phototrophs. Here, we extracted and purified the air-oxidized RfxAc from the photoheterotrophically grown Roseiflexus castenholzii, and we illustrated the structural basis underlying its electron transferring features. Spectroscopic and enzymatic analyses demonstrated the reduction of air-oxidized RfxAc by the ACIII upon oxidation of menaquinol-4 and menaquinol-7. Crystal structures of the air-oxidized and Na-dithionite-reduced RfxAc at 2.2 and 2.0 A resolutions, respectively, showed that the copper ions are coordinated by His77, His146, Cys141, and Met151 in minor different geometries. The Cu(1)-S(delta) bond length increase of Met151, and the electron density Fourier differences at Cu(1) and His77 demonstrated their essential roles in the dithionite-induced reduction. Structural comparisons further revealed that the RfxAc contains a Chloroflexus aurantiacus Ac-A-like copper binding pocket and a hydrophobic patch surrounding the exposed edge of His146 imidazole, as well as an Ac-B-like Ser- and Thr-rich polar patch located at a different site on the surface. These spectroscopic and structural features allow RfxAc to mediate electron transfers between the ACIII and redox partners different from those of Ac-A and Ac-B. These results provide a structural basis for further investigating the electron transfer and energy transformation mechanism of bacterial photosynthesis, and the diversity and evolution of electron transport chains. | ||
| - | + | Structural basis underlying the electron transfer features of a blue copper protein auracyanin from the photosynthetic bacterium Roseiflexus castenholzii.,Wang C, Xin Y, Min Z, Qi J, Zhang C, Xu X Photosynth Res. 2020 Jan 13. pii: 10.1007/s11120-020-00709-y. doi:, 10.1007/s11120-020-00709-y. PMID:31933173<ref>PMID:31933173</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | + | <div class="pdbe-citations 6l9s" style="background-color:#fffaf0;"></div> | |
| - | [[Category: Min, Z | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Min, Z Z]] | ||
[[Category: Wang, C]] | [[Category: Wang, C]] | ||
| - | [[Category: Zhang, C | + | [[Category: Xu, X L]] |
| + | [[Category: Zhang, C Y]] | ||
| + | [[Category: Auracyanin]] | ||
| + | [[Category: Blue copper protein]] | ||
| + | [[Category: Electron transfer]] | ||
| + | [[Category: Metal binding protein]] | ||
| + | [[Category: Na-dithionite reduced]] | ||
| + | [[Category: Roseiflexus castenholzii]] | ||
Revision as of 15:29, 29 January 2020
Crystal structure of Na-dithionite reduced auracyanin from photosynthetic bacterium Roseiflexus castenholzii
| |||||||||||
