1af5

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[[Image:1af5.gif|left|200px]]
[[Image:1af5.gif|left|200px]]
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{{Structure
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{{STRUCTURE_1af5| PDB=1af5 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af5 OCA], [http://www.ebi.ac.uk/pdbsum/1af5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1af5 RCSB]</span>
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'''GROUP I MOBILE INTRON ENDONUCLEASE'''
'''GROUP I MOBILE INTRON ENDONUCLEASE'''
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[[Category: Stephens, K M.]]
[[Category: Stephens, K M.]]
[[Category: Stoddard, B L.]]
[[Category: Stoddard, B L.]]
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[[Category: chloroplast dna]]
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[[Category: Chloroplast dna]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: group i mobile intron]]
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[[Category: Group i mobile intron]]
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[[Category: intron homing]]
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[[Category: Intron homing]]
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[[Category: laglidadg motif]]
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[[Category: Laglidadg motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:38:56 2008''
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Revision as of 07:11, 2 May 2008

Image:1af5.gif

Template:STRUCTURE 1af5

GROUP I MOBILE INTRON ENDONUCLEASE


Overview

The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.

About this Structure

1AF5 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

The structure of I-Crel, a group I intron-encoded homing endonuclease., Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1997 Jun;4(6):468-76. PMID:9187655 Page seeded by OCA on Fri May 2 10:11:15 2008

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