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1af5
From Proteopedia
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[[Image:1af5.gif|left|200px]] | [[Image:1af5.gif|left|200px]] | ||
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'''GROUP I MOBILE INTRON ENDONUCLEASE''' | '''GROUP I MOBILE INTRON ENDONUCLEASE''' | ||
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[[Category: Stephens, K M.]] | [[Category: Stephens, K M.]] | ||
[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
| - | [[Category: | + | [[Category: Chloroplast dna]] |
| - | [[Category: | + | [[Category: Endonuclease]] |
| - | [[Category: | + | [[Category: Group i mobile intron]] |
| - | [[Category: | + | [[Category: Intron homing]] |
| - | [[Category: | + | [[Category: Laglidadg motif]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:11, 2 May 2008
GROUP I MOBILE INTRON ENDONUCLEASE
Overview
The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.
About this Structure
1AF5 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
Reference
The structure of I-Crel, a group I intron-encoded homing endonuclease., Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1997 Jun;4(6):468-76. PMID:9187655 Page seeded by OCA on Fri May 2 10:11:15 2008
