6klt
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Troponin of cardiac thin filament in low-calcium state== | |
| + | <StructureSection load='6klt' size='340' side='right'caption='[[6klt]], [[Resolution|resolution]] 12.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6klt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KLT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6klt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klt OCA], [http://pdbe.org/6klt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klt RCSB], [http://www.ebi.ac.uk/pdbsum/6klt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TNNC1_MOUSE TNNC1_MOUSE]] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [[http://www.uniprot.org/uniprot/TNNI3_MOUSE TNNI3_MOUSE]] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [[http://www.uniprot.org/uniprot/TNNT2_MOUSE TNNT2_MOUSE]] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system. | ||
| - | + | Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.,Oda T, Yanagisawa H, Wakabayashi T J Struct Biol. 2020 Jan 16:107450. doi: 10.1016/j.jsb.2020.107450. PMID:31954841<ref>PMID:31954841</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6klt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Oda, T]] | ||
| + | [[Category: Wakabayashi, T]] | ||
| + | [[Category: Yanagisawa, H]] | ||
| + | [[Category: Cardiac thin filament]] | ||
| + | [[Category: Contractile protein]] | ||
Revision as of 16:09, 29 January 2020
Troponin of cardiac thin filament in low-calcium state
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