6jx7
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of spike protein of feline infectious peritonitis virus strain UU4== | |
| + | <StructureSection load='6jx7' size='340' side='right'caption='[[6jx7]], [[Resolution|resolution]] 3.31Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jx7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Fipv Fipv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JX7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jx7 OCA], [http://pdbe.org/6jx7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jx7 RCSB], [http://www.ebi.ac.uk/pdbsum/6jx7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jx7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment. Here we report a 3.3-A cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry. Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM. Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures. The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation. These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP. | ||
| - | + | Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.,Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW, Hsu SD Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1438-1446. doi:, 10.1073/pnas.1908898117. Epub 2020 Jan 3. PMID:31900356<ref>PMID:31900356</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6jx7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Fipv]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Draczkowski, P]] | ||
| + | [[Category: Hsu, S T.D]] | ||
| + | [[Category: Ko, T P]] | ||
| + | [[Category: Yang, T J]] | ||
| + | [[Category: Cov spike protein]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 08:59, 5 February 2020
Cryo-EM structure of spike protein of feline infectious peritonitis virus strain UU4
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