6lry

From Proteopedia

(Difference between revisions)

Revision as of 04:15, 13 February 2020

Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b

Structural highlights

6lry is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[EDNRB_HUMAN] Hirschsprung disease;Waardenburg-Shah syndrome. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. Defects in EDNRB are associated with Waardenburg syndrome 2, with ocular albinism, autosomal recessive: A disorder characterized by the association of features typical of Waardenburg syndrome type 2 with ocular albinism. Patients manifest reduced visual acuity, albinotic fundus, deafness, hypomelanosis.^[1]

Function

[EDNRB_HUMAN] Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.^[2] [SRTX_ATREN] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.^[3] Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).^[4]

Publication Abstract from PubMed

Sarafotoxins (SRTXs) are endothelin-like peptides extracted from snake venom. SRTXs stimulate the endothelin ETA and ETB receptors and enhance vasoconstriction, followed by left ventricular dysfunction and bronchoconstriction. SRTXs include four major isopeptides, S6a-d, with different subtype selectivities. Here, we report the crystal structure of the human ETB receptor in complex with the non-selective sarafotoxin S6b at 3.0 A resolution. This structure reveals the similarities and differences between the binding modes of the endothelins and S6b. Moreover, molecular dynamics simulations based on the S6b-bound receptor provides structural insight into the subtype selectivity of the sarafotoxins. Our study clarifies the recognition mechanism of the endothelin-like peptide families.

Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b.,Izume T, Miyauchi H, Shihoya W, Nureki O Biochem Biophys Res Commun. 2020 Jan 28. pii: S0006-291X(20)30021-8. doi:, 10.1016/j.bbrc.2019.12.091. PMID:32001000^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Issa S, Bondurand N, Faubert E, Poisson S, Lecerf L, Nitschke P, Deggouj N, Loundon N, Jonard L, David A, Sznajer Y, Blanchet P, Marlin S, Pingault V. EDNRB mutations cause Waardenburg syndrome type II in the heterozygous state. Hum Mutat. 2017 May;38(5):581-593. doi: 10.1002/humu.23206. Epub 2017 Mar 15. PMID:28236341 doi:http://dx.doi.org/10.1002/humu.23206
↑ Webb ML, Chao CC, Rizzo M, Shapiro RA, Neubauer M, Liu EC, Aversa CR, Brittain RJ, Treiger B. Cloning and expression of an endothelin receptor subtype B from human prostate that mediates contraction. Mol Pharmacol. 1995 Apr;47(4):730-7. PMID:7536888
↑ Mourier G, Hajj M, Cordier F, Zorba A, Gao X, Coskun T, Herbet A, Marcon E, Beau F, Delepierre M, Ducancel F, Servent D. Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension. Biochimie. 2011 Aug 29. PMID:21889567 doi:10.1016/j.biochi.2011.08.014
↑ Mourier G, Hajj M, Cordier F, Zorba A, Gao X, Coskun T, Herbet A, Marcon E, Beau F, Delepierre M, Ducancel F, Servent D. Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension. Biochimie. 2011 Aug 29. PMID:21889567 doi:10.1016/j.biochi.2011.08.014
↑ Izume T, Miyauchi H, Shihoya W, Nureki O. Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b. Biochem Biophys Res Commun. 2020 Jan 28. pii: S0006-291X(20)30021-8. doi:, 10.1016/j.bbrc.2019.12.091. PMID:32001000 doi:http://dx.doi.org/10.1016/j.bbrc.2019.12.091

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6lry"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6lry is ON HOLD
+==Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b==
+<StructureSection load='6lry' size='340' side='right'caption='[[6lry]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6lry]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LRY FirstGlance]. <br>
+</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lry OCA], [http://pdbe.org/6lry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lry RCSB], [http://www.ebi.ac.uk/pdbsum/6lry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lry ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[[http://www.uniprot.org/uniprot/EDNRB_HUMAN EDNRB_HUMAN]] Hirschsprung disease;Waardenburg-Shah syndrome. The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.  Defects in EDNRB are associated with Waardenburg syndrome 2, with ocular albinism, autosomal recessive: A disorder characterized by the association of features typical of Waardenburg syndrome type 2 with ocular albinism. Patients manifest reduced visual acuity, albinotic fundus, deafness, hypomelanosis.<ref>PMID:28236341</ref>
+== Function ==
+[[http://www.uniprot.org/uniprot/EDNRB_HUMAN EDNRB_HUMAN]] Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.<ref>PMID:7536888</ref>  [[http://www.uniprot.org/uniprot/SRTX_ATREN SRTX_ATREN]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.<ref>PMID:21889567</ref>   Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).<ref>PMID:21889567</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sarafotoxins (SRTXs) are endothelin-like peptides extracted from snake venom. SRTXs stimulate the endothelin ETA and ETB receptors and enhance vasoconstriction, followed by left ventricular dysfunction and bronchoconstriction. SRTXs include four major isopeptides, S6a-d, with different subtype selectivities. Here, we report the crystal structure of the human ETB receptor in complex with the non-selective sarafotoxin S6b at 3.0 A resolution. This structure reveals the similarities and differences between the binding modes of the endothelins and S6b. Moreover, molecular dynamics simulations based on the S6b-bound receptor provides structural insight into the subtype selectivity of the sarafotoxins. Our study clarifies the recognition mechanism of the endothelin-like peptide families.
-Authors: Izume, T., Miyauchi, H., Shihoya, W., Nureki, O.
+Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b.,Izume T, Miyauchi H, Shihoya W, Nureki O Biochem Biophys Res Commun. 2020 Jan 28. pii: S0006-291X(20)30021-8. doi:, 10.1016/j.bbrc.2019.12.091. PMID:32001000<ref>PMID:32001000</ref>
-Description: Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Nureki, O]]
+<div class="pdbe-citations 6lry" style="background-color:#fffaf0;"></div>
-[[Category: Miyauchi, H]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Lysozyme]]
 [[Category: Izume, T]]
+[[Category: Miyauchi, H]]
+[[Category: Nureki, O]]
 [[Category: Shihoya, W]]
+[[Category: Alpha helical]]
+[[Category: Membrane protein]]
+[[Category: Signaling protein]]

6lry

From Proteopedia

Revision as of 04:15, 13 February 2020

Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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